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Q. No.What is incorrect regarding DNA replication?
1.
For a cell to divide, it must first replicate its DNA.
2.
DNA replication is an all-or-none process; once replication begins, it proceeds to completion.
3.
DNA replication (DNA amplification) cannot be performed in vitro (artificially, outside a cell).
4.
Once replication is complete, it does not occur again in the same cell cycle.
Subtopic: Polymerase Chain Reaction: PCR |
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To isolate DNA from a cell, the cell wall needs to be broken. Match the enzymes in Column I with the type of cell in Column II and select the correct match from the codes given:
Codes:
| Column I | Column II | ||
| A | Lysozyme | P | Fungal cell |
| B | Cellulase | Q | Plant cell |
| C | Chitinase | R | Bacterial cell |
| A | B | C | |
| 1. | P | Q | R |
| 2. | P | R | Q |
| 3. | R | P | Q |
| 4. | R | Q | P |
Subtopic: Host & Desired DNA |
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From NCERT
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Consider the two statements:
| I: | Agar Gel electrophoresis is useful in analysis of DNA. |
| II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Separation and Isolation of DNA fragments |
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Consider the given two statements:
I. It is beneficial to have a multiple cloning site (MCS) within the lac Z gene.
II. When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors.
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
I. It is beneficial to have a multiple cloning site (MCS) within the lac Z gene.
II. When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors.
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic: Cloning Vector |
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Consider the following two statements:
| I: | Plasmid can be used as a vector in recombinant DNA technology experiments. |
| II: | Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Tools: Vectors: I | Cloning Vector |
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| Assertion (A): | Thermostable DNA dependent DNA polymerase is used in Polymerase Chain Reaction |
| Reason (R): | Such enzymes do not require primers and can initiate DNA synthesis |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Polymerase Chain Reaction: PCR |
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| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. |
| Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
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| Assertion (A): | When constructing rDNA, the vector DNA should never be cut by the same restriction endonuclease that was used to cut the donor DNA |
| Reason (R): | Use of same restriction endonuclease will generate same kind of sticky ends retarding the function of DNA ligase. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of the (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
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What is true regarding Downstream Processing?
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Large Scale Production |
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In 1970, Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme that was:
| 1. | EcoR I | 2. | Hind II |
| 3. | Bam HI | 4. | Sma I |
Subtopic: Restriction Enzymes - Main Enzymes |
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