In genetic engineering, the antibiotic-resistance gene is used:
1.  To select healthy vectors
2.  As sequences from where replication starts
3.  To keep the cultures free of infection
4.  As selectable markers

Subtopic:  Selectable Markers |
 84%
From NCERT
AIPMT - 2012

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Which of the following enzymes are used to join bits of DNA:

1. Ligase

2. Primase

3. DNA polymerase

4. Endonuclease

Subtopic:  Tools: Enzymes: II |
 97%
From NCERT
AIPMT - 2002

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Identify the wrong statement with regards to restriction enzyme:

1. They cut the strength at palindromic sites.
2. They are useful in genetic engineering.
3. Sticky ends can be joined by using DNA ligases.
4. Each restriction enzyme functions by inspecting the length of a DNA sequence.

Subtopic:  Restriction Enzymes - Main Enzymes |
From NCERT
NEET - 2020

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Pathogenic bacteria gain resistance to antibiotics due to changes in their :
1.Cosmids2.Plasmids
3.Nucleus4.Nucleoid
Subtopic:  Process of Biotech |
 87%
From NCERT
NEET - 2022

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Bacteria can protect themselves from getting infected by bacteriophages as they synthesize:
1. Methylase
2. Ligases
3. Plasmids
4. Endonucleases

Subtopic:  Restriction Enzymes - Main Enzymes |
From NCERT
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‘Competence’ can be induced in a bacterium by treating them with:
1. Polyethylene glycol
2. A specific divalent cation
3. Colchicine
4. Alkaline phosphatase

Subtopic:  Transforming Plant & Animal Cell |
 76%
From NCERT
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Match the following enzymes with their functions:
 

Column-I

Column-II

(a)

Restriction

endonuclease

(i)

joins the DNA fragments

(b)

Exonuclease

(ii)

extends primers on genomic DNA template

(c)

DNA ligase

(iii)

cuts DNA at a specific position

(d)

Tag polymerase

(iv)

removes nucleotides from the ends of DNA


Select the correct option from the following:

Options: (a) (b) (c) (d)
1. (iii) (i) (iv) (ii)
2. (iii) (iv) (i) (ii)
3. (iv) (iii) (i) (ii)
4. (ii) (iv) (i) (iii)

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II | Tools |
 90%
From NCERT
NEET - 2019

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DNA fragments are 
1. Negatively charged
2. Neutral
3. Either positively or negatively charged depending on their size.
4. Positively charged. 

Subtopic:  Separation and Isolation of DNA fragments |
 93%
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Which of the following is not a feature of the plasmids? 
1. Independent replication
2. Circular structure
3. Transferable
4. Single-stranded 

Subtopic:  Cloning Vector |
 80%
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The cutting of DNA at specific locations became possible with the discovery of 
1. Ligases
2. Restriction enzymes
3. Probes
4. Selectable markers 

Subtopic:  Restriction Enzymes - Main Enzymes |
 94%
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