Q. No.Downstream processing involves all the following except:
1.
Separation of the product
2.
Purification of the product
3.
Testing for quality
4.
Biosynthesis
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Regarding competence for bacterial cell transformation:
| I: | Natural competence is not seen in bacterial cells as DNA is hydrophilic and a large molecule. |
| II: | Competence can be induced in bacterial cells with the help of divalent cations and brief heat shock. |
1. Only I is correct.
2. Only II is correct.
3. Both I and II are correct.
4. Both I and II are incorrect.
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A circular plasmid of 10000 bp is digested with two restriction enzymes A and B, to produce 3000 bp and 2000 bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A [A1] is present at the 100th base, the order in which the remaining sites [A2, B1 and B2] are present is:
| 1. | 5100, 3100, 8100 | 2. | 3100, 5100, 8100 |
| 3. | 8100, 5100, 3100 | 4. | 8100, 3100, 5100 |
The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase
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To isolate DNA in pure form from a bacterial cell, it should initially be treated with:
| 1. | Proteases and RNase but not DNase | 2. | Lysozyme |
| 3. | Restriction endonuclease | 4. | DNA ligase |
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DNA fragments resulting from restriction enzyme digestion are moved through agar gel during electrophoresis. Which of the following modifications to the fragments will not affect the rate of their movement?
1. Neutralizing the negative charge
2. Increasing length
3. Changing the base composition
4. Decreasing length
The enzyme DNA ligase, important in rDNA procedures, catalyzes the formation of:
| 1. | N- Glycosidic | 2. | Hydrogen |
| 3. | Phosphodiester | 4. | Ester |
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Consider the two statements:
| I: | It is beneficial to have a multiple cloning site (MCS) within the lac Z gene of the plasmid of E.coli. |
| II: | This allows the researcher to distinguish between recombinant and non-recombinant vectors if X-gal is added to the medium. |
1. Both I and II are correct and II explains I.
2. Both I and II are correct but II does not explain I.
3. I is correct but II is incorrect.
4. Both I and II are incorrect.
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The trait ‘resistance to certain antibiotics’ is seen in some bacteria due to the presence in them of:
| 1. | Inclusion bodies |
| 2. | Membranous extensions of plasma membrane |
| 3. | Plasmid DNA |
| 4. | Glycocalyx |
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‘Downstream processing’ does not include:
| 1. | Product biosynthesis | 2. | Product isolation |
| 3. | Product purification | 4. | Product polishing |
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