Q. No.Regarding competence for bacterial cell transformation:
I:
Natural competence is not seen in bacterial cells as DNA is hydrophilic and a large molecule.
II:
Competence can be induced in bacterial cells with the help of divalent cations and brief heat shock.
1. Only I is correct.
2. Only II is correct.
3. Both I and II are correct.
4. Both I and II are incorrect.
2. Only II is correct.
3. Both I and II are correct.
4. Both I and II are incorrect.
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Downstream processing involves all the following except:
| 1. | Separation of the product | 2. | Purification of the product |
| 3. | Testing for quality | 4. | Biosynthesis |
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A circular plasmid of 10000 bp is digested with two restriction enzymes A and B, to produce 3000 bp and 2000 bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A [A1] is present at the 100th base, the order in which the remaining sites [A2, B1 and B2] are present is:
| 1. | 5100, 3100, 8100 | 2. | 3100, 5100, 8100 |
| 3. | 8100, 5100, 3100 | 4. | 8100, 3100, 5100 |
The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase
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To isolate DNA in pure form from a bacterial cell, it should initially be treated with:
| 1. | Proteases and RNase but not DNase | 2. | Lysozyme |
| 3. | Restriction endonuclease | 4. | DNA ligase |
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DNA fragments resulting from restriction enzyme digestion are moved through agar gel during electrophoresis. Which of the following modifications to the fragments will not affect the rate of their movement?
1. Neutralizing the negative charge
2. Increasing length
3. Changing the base composition
4. Decreasing length
The enzyme DNA ligase, important in rDNA procedures, catalyzes the formation of:
| 1. | N- Glycosidic | 2. | Hydrogen |
| 3. | Phosphodiester | 4. | Ester |
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| 1. | For a cell to divide, it must first replicate its DNA. |
| 2. | DNA replication is an all-or-none process; once replication begins, it proceeds to completion. |
| 3. | DNA replication (DNA amplification) cannot be performed in vitro (artificially, outside a cell). |
| 4. | Once replication is complete, it does not occur again in the same cell cycle. |
| Column I | Column II | ||
| A | Lysozyme | P | Fungal cell |
| B | Cellulase | Q | Plant cell |
| C | Chitinase | R | Bacterial cell |
| A | B | C | |
| 1. | P | Q | R |
| 2. | P | R | Q |
| 3. | R | P | Q |
| 4. | R | Q | P |
| I: | Agar Gel electrophoresis is useful in analysis of DNA. |
| II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
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