Q. No.Plasmid pUC18 carries lacZ gene that confers the host cells to convert white X-gal into blue indole. A gene is inserted within the lacZ gene of the plasmid. Bacterial cells transformed with this rDNA, if provided with X-gal in the medium, will produce:
1.
Blue colonies
2.
White colonies
3.
No colonies
4.
Orange colonies if seen through UV light
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The underlying basis of the Polymerase Chain Reaction is:
| 1. | DNA synthesis and hence amplification can occur in a template independent manner. |
| 2. | DNA, being thermostable, can be amplified during the thermal cycle in PCR. |
| 3. | DNA polymerase I can synthesize large amounts of DNA. |
| 4. | PCR allows the direct amplification of DNA even if it is present in minute quantities. |
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DNA fragments digested by restriction endonucleases separate on agar gel electrophoresis according to their size because:
| 1. | they are forced to move towards cathode under an electric field |
| 2. | agar gel provides a sieving effect |
| 3. | larger fragments will have more negative charge and will move farther in the electric field |
| 4. | chelation with ethidium bromide reduce the rate of transport of fragments |
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Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies
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The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
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Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
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The Ti plasmid, used in transformation of plant cells, is found in:
| 1. | Agrobacterium tumefaciens | 2. | Escherichia coli |
| 3. | Bacillus thuringiensis | 4. | Xanthomonas citri |
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Identify the incorrect statement regarding restriction endonucleases:
| 1. | They mostly cut dsDNA at specific base sequences. |
| 2. | They are produced by bacterial cells as a mechanism of self-defence. |
| 3. | They digest DNA by removing nucleotides from a free 3' end. |
| 4. | They often generate short single-stranded sequences at the ends of the resultant fragments. |
Bioprocessing engineering deals with:
| 1. | the search for plant and animal species from which medicinal drugs and other commercially valuable compounds can be obtained. |
| 2. | developing methods and software tools for understanding biological data. |
| 3. | production of active pharmaceutical substances in genetically modified organisms (GMOs). |
| 4. | maintenance of sterile ambience in chemical engineering processes. |
Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
| 1. | wheat | 2. | soybean |
| 3. | rice | 4. | barley |
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