Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:

1.  wheat 2.  soybean
3.  rice 4.  barley

Subtopic:  Transforming Plant & Animal Cell |
 68%

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The steps included in a cycle of Polymerase Chain Reaction are:
1. initiation, elongation, and termination
2. transfection, transformation, and transduction
3. denaturation, annealing, and extension
4. restriction, electrophoresis, and elution

Subtopic:  Polymerase Chain Reaction: PCR |
 93%

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Consider the two statements:

I:  It is desirable to use restriction enzymes produce 'sticky ends' for cloning experiments in genetic engineering.
II:  If the donor DNA and the vector DNA are cut by such same restriction endonuclease, ligation of the two is easy.
 
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. Only I is correct
4. Both I and II are incorrect
Subtopic:  Restriction Enzymes - Main Enzymes |
 72%

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If you want to isolate DNA in pure form from a fungal cell, the first enzyme required normally would be:

1.  Restriction endonuclease 2.  DNA ligase
3.  DNA polymerase 4.  Chitinase
Subtopic:  Host & Desired DNA: I | Host & Desired DNA: II | Transforming Plant & Animal Cell |
 87%
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In agarose gel electrophoresis, the DNA fragments produced by restriction enzyme digestion:

1.  will move towards anode with smaller fragments moving farther than the larger segments
2.  will move towards anode with larger fragments moving farther than the smaller segments
3.  will move towards cathode with smaller fragments moving farther than the larger segments
4.  will move towards cathode with larger fragments moving farther than the smaller segments
Subtopic:  Process of Biotech |
 86%
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In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:

1. 1 and 2
2. 3 and 4
3. 6 and 7
4. 5 and 8
Subtopic:  Cloning Vector |
 90%
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What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?

1. Ori 2. rop
3. Selectable markers 4. Promoter in the insert
Subtopic:  Cloning Vector | Obtaining Copy of Gene from Donor DNA |
 86%
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The ability of plasmids and bacteriophage DNA to replicate within bacterial cells:

1.  depends on a particular or sequence of the main chromosomal DNA
2.  is possible due to promoter sequence present in the insert
3.  cannot occur without telomeric sequences
4.  is independent of the control of chromosomal DNA
Subtopic:  Cloning Vector |
 58%
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The construction of first rDNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of:

1. Salmonella typhimurium 2. Escherechia coli
3. Agrobacterium tumefaciens 4. Haemophilus influenzae
Subtopic:  Restriction Enzymes - Main Enzymes | Cloning Vector |
 75%
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The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?

1. Banting and Best 2. Bolivar and Rodriguez
3. Hershey and Chase 4. Cohen and Boyer
Subtopic:  Restriction Enzymes - Main Enzymes |
 84%
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