Q. No.Consider the two statements:
I:
It is desirable to use restriction enzymes produce 'sticky ends' for cloning experiments in genetic engineering.
II:
If the donor DNA and the vector DNA are cut by such same restriction endonuclease, ligation of the two is easy.
1.
Both I and II are correct and II explains I
2.
Both I and II are correct but II does not explain I
3.
Only I is correct
4.
Both I and II are incorrect
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The steps included in a cycle of Polymerase Chain Reaction are:
1. initiation, elongation, and termination
2. transfection, transformation, and transduction
3. denaturation, annealing, and extension
4. restriction, electrophoresis, and elution
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Agrobacterium tumefaciens can be an effective vector for delivering genes of interest into the cells of:
| 1. | wheat | 2. | soybean |
| 3. | rice | 4. | barley |
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In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
|
 |
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What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
| 1. | Ori | 2. | rop |
| 3. | Selectable markers | 4. | Promoter in the insert |
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The work of which of the following scientists led to the establishment of the discipline of modern biotechnology?
| 1. | Banting and Best | 2. | Bolivar and Rodriguez |
| 3. | Hershey and Chase | 4. | Cohen and Boyer |
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A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
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What limitation of traditional hybridization procedures used in plant and animal breeding can be overcome by recombinant DNA procedures?
| I: | Inclusion and multiplication of undesirable genes |
| II: | Inability of inclusion of desirable traits from other species |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
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A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3.Beta Galactosidase
4. Chloramphenicol acetyl transferase
Identify (a) and (b) bioreactors respectively in the given diagram:
1. Simple stirred-tank and sparged stirred – tank
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift
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