Q. No.Restriction endonuclease
1. Cuts the DNA molecule randomly
2. Cuts the DNA molecule at specific sites
3. Restricts the synthesis of DNA inside the nucleus.
4. Synthesizes DNA.
1. Cuts the DNA molecule randomly
2. Cuts the DNA molecule at specific sites
3. Restricts the synthesis of DNA inside the nucleus.
4. Synthesizes DNA.
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Two microbes found to be very useful in gene engineering are
1. Escherichia coli and Agrobacterium tumefaciens.
2. Vibrio cholerae and a tailed bacteriophage.
3. Diplococcus sp. And Pseudomonas sp.
4. Crown gall bacterium and Caenorphaditiselegans.
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Gel electrophoresis is used for
1. Isolation of DNA molecules.
2. Cutting of DNA into fragments.
3. Separation of DNA fragments according to their size.
4. Construction of recombinant DNA by joining with cloning vector.
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Polyethylene glycol method is used for
1. Energy production from sewage.
2. Gene transfer without a vector
3. Biodiesel production.
4. Seedless fruit production.
Which one of the following is used as a vector for cloning genes into higher organisms?
1. Retrovirus
2. Baculovirus
3. Salmonella typhimurium
4. Rhizopusnigricans
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Restriction endonucleases are enzymes which
1. Remove nucleotides from the ends of the DNA molecule.
2. Make cuts at specific positions within the DNA molecule.
3. Recognize a specific nucleotide sequence for binding of DNA ligase.
4. Restrict the action of the enzyme DNA polymerase.
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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
1. 5’______CACGTA_______3’
3’______CTVAGT_______5’
2. 5’______CGTTCG_______3’
3’______ATGGTA_______5’
3. 5’______GATATG_______3’
3’______CTACTA_______5’
4. 5’______GAATTC_______3’
3’______CTTAAG_______5’
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Which of the following are used in gene cloning?
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleotides
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Which vector can clone only a small fragment of DNA?
1. Bacterial artificial chromosomes
2. Yeast artificial chromosomes
3. Plasmid
4. Cosmid
The cutting of DNA at specific locations became possible with the discovery of
1. Ligases
2. Restriction enzymes
3. Probes
4. Selectable markers
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