The following palindrome is recognized by the restriction enzyme:

 

1.  BamHI 2.  EcoRI
3.  HindII 4.  PstI

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
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pBR322 does not contain the site for:

1.  Pvu I 2. BamH I
3.  Sma I 4. EcoR I
Subtopic:  Cloning Vector |
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A method used only for transforming animal cells is:

1. Biolistics 2. Microinjection
3. Use of virus 4. Agrobacterium mediation
Subtopic:  Host & Desired DNA |
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Lysozyme should be used when isolating DNA in a pure form from:

1. A bacterial cell 2. A fungal cell
3. A plant cell 4. An animal cell
Subtopic:  Process of Biotech | Restriction Enzymes - Main Enzymes | General Design of an rDNA experiment |
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In recombinant DNA experiments, a vector:

1.  carries DNA into a new cell 
2.  links together newly joined fragments of DN
3.  makes millions of copies of a specific segment of DNA
4.  separates fragments of DNA by their length and electrical charges

Subtopic:  Cloning Vector |
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If any protein-encoding gene is expressed in a heterologous host, the protein formed is called:

1.  Recombinant protein 2.  Native protein
3.  Pro-protein 4.  Exotic protein
Subtopic:  Tools |
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Gene cloning occurs:

1.  when a phage transfers bacterial DNA from one bacterium to another.
2.  when a bacterium takes up DNA from the surrounding fluid.
3.  when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. 
4.  when DNA is produced from an RNA template.

Subtopic:  Process of Biotech |
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Identify (a) and (b) bioreactors respectively in the given diagram:

 

1. Simple stirred-tank and sparged stirred – tank 
2. Sparged stirred - tank and simple stirred-tank
3. Airlift and tower
4. Tower and airlift

Subtopic:  Tools |
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Which among the following is a cloning vector?

1.  EcoRI 2.  BamHI
3.  pUC18 4. Sal I
Subtopic:  Cloning Vector |
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A reporter enzyme used in biotechnology includes all the following except:
1. Green Fluorescent Protein
2. Luciferase
3.Beta Galactosidase
4. Chloramphenicol acetyl transferase

Subtopic:  Tools |

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