Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
1. | Laboratory flask of the largest capacity |
2. | A stirred-tank bioreactor without in-lets and out-lets |
3. | A continuous culture system |
4. | Any of the above |
Which of the following statements does not hold true for restriction enzyme?
1. | It recognises a palindromic nucleotide sequence |
2. | It is an endonuclease |
3. | It is isolated from viruses |
4. | It can produce the same kind of sticky ends in different DNA molecules |
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Restriction endonucleases present in bacterial cells provide an advantage to the cell because these enzymes:
1. | can identify the mutated sequences on the chromosomal DNA and help in their repair |
2. | selectively bind to particular nucleotide sequences that may appear in viral DNA preventing its replication in the cell |
3. | can cut the plasmid DNA of the bacterial cell that allows them to recombine foreign DNA into their plasmid DNA |
4. | help the bacterium take up foreign DNA from their environment and combine it into their genome |
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
1. | Telomeric sequence | 2. | Multiple cloning sites |
3. | Ori | 4. | Selectable marker |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
1. | EcoRI | 2. | HindII |
3. | SmaI | 4. | BamHI |
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The following palindrome is recognized by the restriction enzyme:
1. | BamHI | 2. | EcoRI |
3. | HindII | 4. | PstI |
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pBR322 does not contain the site for:
1. | Pvu I | 2. | BamH I |
3. | Sma I | 4. | EcoR I |
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A method used only for transforming animal cells is:
1. | Biolistics | 2. | Microinjection |
3. | Use of virus | 4. | Agrobacterium mediation |
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Lysozyme should be used when isolating DNA in a pure form from:
1. | A bacterial cell | 2. | A fungal cell |
3. | A plant cell | 4. | An animal cell |
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In recombinant DNA experiments, a vector:
1. | carries DNA into a new cell |
2. | links together newly joined fragments of DN |
3. | makes millions of copies of a specific segment of DNA |
4. | separates fragments of DNA by their length and electrical charges |
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