Q. No.Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
1.
DNA can be seen in visible light
2.
DNA can be seen without staining in visible light
3.
Ethidium bromide stained DNA can be seen in visible light
4.
Ethidium bromide stained DNA can be seen under exposure to UV light
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of the phosphodiester bonds in DNA by the enzyme |
| 2. | Cutting of DNA at a specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
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Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
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Which of the following should be chosen for the best yield if one were to produce a recombinant protein in large amounts?
| 1. | Laboratory flask of the largest capacity |
| 2. | A stirred-tank bioreactor without in-lets and out-lets |
| 3. | A continuous culture system |
| 4. | Any of the above |
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
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The first type II restriction endonuclease whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoRI | 2. | HindII |
| 3. | SmaI | 4. | BamHI |
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The following palindrome is recognized by the restriction enzyme:
| 1. | BamHI | 2. | EcoRI |
| 3. | HindII | 4. | PstI |
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A method used only for transforming animal cells is:
| 1. | Biolistics | 2. | Microinjection |
| 3. | Use of virus | 4. | Agrobacterium mediation |
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Lysozyme should be used when isolating DNA in a pure form from:
| 1. | A bacterial cell | 2. | A fungal cell |
| 3. | A plant cell | 4. | An animal cell |
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