Taq polymerase is used in the polymerase chain reaction because:

1.  It replicates DNA faster than other enzymes
2.  It is the only enzyme that can replicate DNA invitro
3.  It does not require an RNA primer to function
4.  It is thermostable

Subtopic:  Polymerase Chain Reaction: PCR |
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A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites

Subtopic:  Cloning Vector |
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Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?

1.endonuclease2.exonuclease
3.DNA ligase4.Hind – II

Subtopic:  Tools: Enzymes: I | Tools: Enzymes: II |
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The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:

1.  Transduction 2.  Conjugation
3.  Transformation 4.  Translation

Subtopic:  Host & Desired DNA: I | Host & Desired DNA: II |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

1.Charge only2.Size only
3.Charge-to-size ratio4.All of the above

Subtopic:  Selectable Markers: I | Selectable Markers: II | Host & Desired DNA | Tools |
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An antibiotic resistance gene in a vector usually helps in the selection of:

1. Competent bacterial cells

2. Transformed bacterial cells

3. Recombinant bacterial cells

4. None of the above

Subtopic:  Selection of Recombinant Transformants |
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:

1.  Binding of DNA to the cell wall
2.  Uptake of DNA through membrane transport proteins
3.  Uptake of DNA through transient pores in the bacterial cell wall
4.  Expression of antibiotic resistance gene

Subtopic:  Host & Desired DNA: I | Host & Desired DNA: II |
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A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:

1.  Human genes may have intron which bacteria cannot process
2.  Amino acid codons for humans and bacteria are different
3.  Human protein is formed but degraded by bacteria
4.  All of the above

Subtopic:  Tools | General Design of an rDNA experiment |
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Who among the following was awarded the Nobel Prize for the development of the PCR technique?

1.Herbert Boyer2.Hargovind Khurana
3.Kary Mullis4.Arthur Kornberg

Subtopic:  Polymerase Chain Reaction: PCR |
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Which of the following cannot be used as a vector in rDNA technology?

1.Plasmid2.Phage DNA
3.Bacterium4.YAC

Subtopic:  Tools |
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