Taq polymerase is used in the polymerase chain reaction because:
1. | It replicates DNA faster than other enzymes |
2. | It is the only enzyme that can replicate DNA invitro |
3. | It does not require an RNA primer to function |
4. | It is thermostable |
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A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?
1. | endonuclease | 2. | exonuclease |
3. | DNA ligase | 4. | Hind – II |
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The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
1. | Transduction | 2. | Conjugation |
3. | Transformation | 4. | Translation |
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
1. | Charge only | 2. | Size only |
3. | Charge-to-size ratio | 4. | All of the above |
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An antibiotic resistance gene in a vector usually helps in the selection of:
1. Competent bacterial cells
2. Transformed bacterial cells
3. Recombinant bacterial cells
4. None of the above
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The significance of the 'heat shock' method in bacterial transformation is to facilitate:
1. | Binding of DNA to the cell wall |
2. | Uptake of DNA through membrane transport proteins |
3. | Uptake of DNA through transient pores in the bacterial cell wall |
4. | Expression of antibiotic resistance gene |
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A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be:
1. | Human genes may have intron which bacteria cannot process |
2. | Amino acid codons for humans and bacteria are different |
3. | Human protein is formed but degraded by bacteria |
4. | All of the above |
Who among the following was awarded the Nobel Prize for the development of the PCR technique?
1. | Herbert Boyer | 2. | Hargovind Khurana |
3. | Kary Mullis | 4. | Arthur Kornberg |
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Which of the following cannot be used as a vector in rDNA technology?
1. | Plasmid | 2. | Phage DNA |
3. | Bacterium | 4. | YAC |
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