If the required gene has been isolated by shotgun approach, care must be taken to:

1.	cut the plasmid with a different restriction enzyme than that used to obtain the donor gene
2.	cut the plasmid with the same restriction enzyme that was used to obtain the donor gene
3.	avoid cutting the plasmid
4.	cut the plasmid with two different restriction enzymes

The chronological steps of a polymerase chain reaction are:

Taq polymerase is used in the polymerase chain reaction because:

A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites

Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?

The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:

In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

An antibiotic resistance gene in a vector usually helps in the selection of:

1. Competent bacterial cells

2. Transformed bacterial cells

3. Recombinant bacterial cells

4. None of the above

The significance of the 'heat shock' method in bacterial transformation is to facilitate:

A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. The reasons could be: