Q. No.A DNA library is:
1.
information in a computer database about all the genes sequenced so far
2.
a collection of vectors in a molecular biology lab
3.
a collection of fragments of DNA that make up the entire genome of an organism
4.
all the restriction sites put together on a DNA fragment
A foreign DNA was introduced at a cloning site within the gene for beta-galactosidase in a plasmid of a bacterium. The bacterium containing this rDNA was plated on a medium containing X-gal. They were distinguished by the fact that they remained white. This method should be called as:
| 1. | selection | 2. | screening |
| 3. | transformation | 4. | transduction |
Cohen and Boyer constructed one of the first genetically engineered chimeras, pSC101. They introduced in this plasmid the gene of Xenopus laevis that coded for:
| 1. | cytochrome c | 2. | haemoglobin |
| 3. | keratin | 4. | rRNA |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
If the required gene has been isolated by shotgun approach, care must be taken to:
| 1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
| 2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
| 3. | avoid cutting the plasmid |
| 4. | cut the plasmid with two different restriction enzymes |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
The chronological steps of a polymerase chain reaction are:
| 1. | Denaturation, Annealing of primers, Primer extension |
| 2. | Annealing of primers, Denaturation, Primer extension |
| 3. | Annealing of primers, Primer extension, Denaturation |
| 4. | Denaturation, Primer extension, Annealing of primers |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
Taq polymerase is used in the polymerase chain reaction because:
| 1. | It replicates DNA faster than other enzymes |
| 2. | It is the only enzyme that can replicate DNA invitro |
| 3. | It does not require an RNA primer to function |
| 4. | It is thermostable |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
A major advantage of using YAC as a cloning vector over the plasmids is that:
1. it can replicate independently
2. it can be selected easily
3. it can accommodate larger inserts
4. it has multiple cloning sites
Which of the following enzymes catalyze the removal of nucleotides from the ends of DNA?
| 1. | endonuclease | 2. | exonuclease |
| 3. | DNA ligase | 4. | Hind – II |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
The transfer of genetic material from one bacterium to another through the mediation of a viral vector is termed as:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
| 1. | Charge only | 2. | Size only |
| 3. | Charge-to-size ratio | 4. | All of the above |
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
To unlock all the explanations of 38 chapters you need to be enrolled in MasterClass Course.
© 2024 GoodEd Technologies Pvt. Ltd.