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Q. No.Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?
| 1. | restriction endonuclease - production of DNA fragments for gene cloning. |
| 2. | DNA polymerase - copies DNA sequences to create primers in the polymerase chain reaction. |
| 3. | reverse transcriptase - production of cDNA from mRNA. |
| 4. | electrophoresis - RLFP analysis. |
The one gene — one enzyme hypothesis basically means that:
1. Every gene encodes a protein.
2. Each structural gene encodes an essential metabolic enzyme.
3. Each structural protein is encoded by a single gene.
4. Genes are always expressed as enzymes
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
| I. | Transform bacteria with recombinant DNA molecule. |
| II. | Cut the plasmid DNA using restriction enzymes. |
| III. | Extract plasmid DNA from bacterial cells. |
| IV. | Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. |
| V. | Use ligase to seal plasmid DNA to non-plasmid DNA. |
1. I, II, IV, III, V
2. II, III, V, IV, I
3. III, II, IV, V, I
4. III, IV, V, I, II
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Identify the correct statement:
| 1. | The structural gene is polycistronic mostly in eukaryotes and monocistronic mostly in prokaryotes. |
| 2. | Introns are said to be those sequences in the eukaryotic structural gene that appear in mature or processed mRNA. |
| 3. | During transcription both strands of the DNA are transcribed but in a short length. |
| 4. | Transcription and translation can be coupled in bacteria. |
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Q. No.
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