Q. No.What process causes shortening of telomeres during cell division?
1. DNA synthesis.
2. Exonuclease removal of primers.
3. Ligation of DNA fragments.
4. Telomerase activity.
1. DNA synthesis.
2. Exonuclease removal of primers.
3. Ligation of DNA fragments.
4. Telomerase activity.
Dideoxyribonucleotide chain-termination is a method of
1. cloning DNA.
2. sequencing DNA.
3. digesting DNA.
4. synthesizing DNA.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
| I. | Transform bacteria with recombinant DNA molecule. |
| II. | Cut the plasmid DNA using restriction enzymes. |
| III. | Extract plasmid DNA from bacterial cells. |
| IV. | Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. |
| V. | Use ligase to seal plasmid DNA to non-plasmid DNA. |
1. I, II, IV, III, V
2. II, III, V, IV, I
3. III, II, IV, V, I
4. III, IV, V, I, II
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Which of the following tools of recombinant DNA technology is INCORRECTLY paired with one of its uses?
| 1. | restriction endonuclease - production of DNA fragments for gene cloning. |
| 2. | DNA polymerase - copies DNA sequences to create primers in the polymerase chain reaction. |
| 3. | reverse transcriptase - production of cDNA from mRNA. |
| 4. | electrophoresis - RLFP analysis. |
The "Southern" technique involves:
| 1. | the detection of DNA fragments on membranes by specific radioactive antibodies. |
| 2. | the detection of proteins on membranes using a radioactive DNA probe. |
| 3. | the detection of DNA fragments on membranes by a radioactive DNA probe. |
| 4. | the detection of proteins on membranes using specific radioactive antibodies. |
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Hybridization of mRNA with DNA can reveal which segment of the DNA are introns and which segment are exons. When the magnification is high enough to see the molecule, the introns appear as
1. Single stranded loops in a double stranded molecule
2. Separate fragments
3. Triple stranded nucleic acid molecules
4. Barr bodies
A piece of nucleic acid used to find a gene, by forming a hybrid with it, is called a
1. Probe
2. Vector
3. Restriction sequence
4. Retrovirus
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The association of histone H1 with a nucleosome indicates:
1. DNA replication is occurring
2. The DNA is condensed into a chromatin fibre
3. The DNA double helix is exposed
4. Transcription is occurring
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The diagram shows an important concept in the genetic implication of DNA. Fill in the blanks A to C.
1. A - translation B - transcription C - Erwin Chargaff
2. A -transcription B - translation C - Francis Crick
3. A - translation B - extension C - Rosalind Franklin
4. A - transcription B - replication C - James Watson
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1. sn RNA
2. hn RNA
3. 23 S rRNA
4. 5 S rRNA
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