Q. No.Name the enzyme that facilitates opening of DNA helix during transcription.
1. DNA helicase
2. DNA polymerase
3. RNA polymerase
4. DNA ligase
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Choose the correct pair from the following :
| 1. | Polymerases | - | Break the DNA into fragments |
| 2. | Nucleases | - | Separate the two strands of DNA |
| 3. | Exonucleases | - | Make cuts at specific positions within DNA |
| 4. | Ligases | - | Join the two DNA molecules |
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What is added during ‘capping’ in post-transcriptional modification of hn RNA?
1. Methyl guanosine triphosphate at 5’ end
2. Methyl guanosine triphosphate at 3’ end
3. Adenylate residues at the 5’ end
4. Adenylate residues at the 3’ end
Identify the correct statement:
| 1. | The structural gene is polycistronic mostly in eukaryotes and monocistronic mostly in prokaryotes. |
| 2. | Introns are said to be those sequences in the eukaryotic structural gene that appear in mature or processed mRNA. |
| 3. | During transcription both strands of the DNA are transcribed but in a short length. |
| 4. | Transcription and translation can be coupled in bacteria. |
During DNA replication the leading strand:
1. is replicated continuously towards the replication fork
2. is replicated discontinuously towards the replication fork
3. is replicated continuously away from the replication fork
4. is replicated continuously away from the replication fork
In eukaryotes, RNA polymerase II transcribes:
1. heterogenous nuclear RNA
2. small nuclear RNA
3. transfer RNA
4. ribosomal RNA
During translation, formation of peptide bonds in bacteria is catalyzed by:
1. Polynucleotide phosphorylase
2. Protease
3. Ligase
4. Ribozyme
What is incorrect regarding the salient features of the DNA double helix?
1. The two chains have anti-parallel polarity
2. The base pairing is complementary
3. Backbone of a strand is constituted by sugar-phosphate
4. The pitch is 0.34 nm
The size of VNTR [used in DNA fingerprinting] varies from:
| 1. | 0.1 to 20 kb | 2. | 20 to 30 kb |
| 3. | 50 to 100 kb | 4. | 115 to 215 kb |
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The predominant site for control of gene expression in prokaryotes is:
1. Transcription initiation
2. Binding of mRNA to ribosome
3. Translation termination
4. Post translational modification
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