How many recognition sequences for the restriction enzyme EcoRI is/are expected to be present in a dsDNA of 10000 base pair length?
1. 0
2. 1
3. 2
4. 4
The template strand of DNA is 3’ – TAC – 5’. The anticodon that will pair the corresponding mRNA codon will be:
1. 5’ – AUG – 3’
2. 5’ – UAC – 3’
3. 5’ – CAU – 3’
4. 3’ – TAC – 5’
Transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization is called as:
1. | DNA profiling | 2. | PCR |
3. | Southern blotting | 4. | Elution |
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Which scientist experimentally proved that DNA is the sole genetic material in bacteriophage?
1. Beadle and Tatum
2. Messelson and Stahl
3. Hershey and Chase
4. Jacob and Monod
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Match the following RNA polymerases with their transcribed products:
Column-I |
Column-II |
||
(a) |
RNA polymerase I |
(i) |
tRNA |
(b) |
RNA polymerase II |
(ii) |
rRNA |
(c) |
RNA polymerase III |
(iii) |
hnRNA |
Select the correct option from the following:
Options: | (a) | (b) | (c) |
1. | i | iii | ii |
2. | i | ii | iii |
3. | ii | iii | i |
4. | iii | ii | i |
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A ribosomal binding site in bacterial and archaeal messenger RNA, generally located around 8 bases upstream of the start codon AUG is called as:
1. Shine-Dalgarno sequence.
2. Kozak consensus sequence.
3. Sorting signals.
4. Prinbow box.
The one gene — one enzyme hypothesis basically means that:
1. Every gene encodes a protein.
2. Each structural gene encodes an essential metabolic enzyme.
3. Each structural protein is encoded by a single gene.
4. Genes are always expressed as enzymes
Mutations can lead to incorporation of wrong amino acid into the polypeptide chain. This is least likely to occur when there is:
1. a change in the wobble base of the codon.
2. a mistake in amino acid attachment to the tRNA.
3. a change in the third base of the anticodon.
4. a gene mutation involving the first base of the codon.
The essential difference between DNA replication in eukaryotes and prokaryotes will be:
1. A high % of AT base pairs in Ori region of eukryotic DNA.
2. Bidirectional movement of replication fork in eukaryotes.
3. Eukaryotes contain several different DNA polymerases.
4. Telomerase required to replicate the ends of eukaryotic chromosomes.
All the following regarding RNA splicing are correct except:
1. Some introns can splice themselves out.
2. Splicing is required for some tRNAs.
3. The final step of splicing is formation of a phosphodiester linkage.
4. Introns often have a specific function after they are removed.
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