Even in the presence of malonate, the velocity of the reaction catalyzed by the enzyme succinic dehydrogenase can be increased by:
1. increasing the temperature beyond 40⁰C
2. decreasing the pH
3. increasing the concentration of succinate
4. decreasing the production of ATP by chemiosmosis

Identify the incorrect statement regarding enzyme action:
1. Formation of [Enzyme Substrate] complex is obligatory
2. Enzymes speed up a chemical reaction by lowering the requirement of activation energy
3. Substrate need not go through a transition state if the enzyme catalyzed reaction is endothermic
4. Enzymes are themselves not used up in the chemical reaction catalyzed by them

Enzymes are biochemical catalysts that:
1. can change the equilibrium position of the reaction it catalyses by lowering the energy of activation of that reaction.
2. can lower the energy of activation of the reaction it catalyses by increasing the molecular collisions between the molecules.
3. lower the free energy difference between substrate(s) and product(s) but it cannot change the equilibrium position of the reaction it catalyses.
4. cannot change the equilibrium position of the reaction it catalyses but it lowers the energy of activation of that reaction.