The earlier technique of DNA fingerprinting involved Southern blot and all the following except:
1. digestion of DNA by deoxyribonuclease
2. separation of DNA fragments by electrophoresis
3. hybridisation using labelled VNTR probe
4. detection of hybridised DNA fragments by autoradiography

Subtopic:  DNA Fingerprinting |
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Which histone is not a part of the octamer that forms the core of histone molecules in a nucleosome?
1. H1
2. H2A
3. H2B
3. H3
Subtopic:  DNA Packaging |
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Consider the two statements:
Assertion (A):   It is necessary to define the boundaries that would demarcate the region and the strand of DNA that would be transcribed.
Reason (R):  In transcription, only a segment of DNA and only one of the strands is copied into RNA.
 
1. Both (A) and (R) are True and (R) is the correct explanation of (A).
2. Both (A) and (R) are True but (R) is not the correct explanation of (A).
3. (A) is True but (R) is False.
4. Both (A) and (R) are False.
Subtopic:  Transcription |
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Consider the two statements:
Assertion (A):  When Griffith injected a mixture of heat-killed S and live R bacteria, the mice died.
Reason (R): Mice infected with the S strain (virulent) die from pneumonia infection but mice infected with the R strain do not develop pneumonia.
 
1. Both (A) and (R) are True and (R) is the correct explanation of (A).
2. Both (A) and (R) are True but (R) is not the correct explanation of (A).
3. (A) is True but (R) is False.
4. Both (A) and (R) are False.
Subtopic:  Search for Genetic Material |
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Regarding DNA replication:
I: It is semi-conservative.
II: It is semi-discontinuous.
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic:  DNA Replication |
 76%
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The RNA polymerase I does not transcribe:
1. 28S rRNA
2. 18S rRNA
3. 5.8S rRNA
4. 5S rRNA

Subtopic:  Transcription |
 81%
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The repressor of the lac operon is synthesised:
 
1. all the time
2. only when lactose is present
3. only when lactose is absent
4. only when glucose is present in high amount
Subtopic:  Gene Regulation: Lac Operon |
 69%
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Consider the two statements:

Assertion (A): Transcription and translation can be coupled in bacteria.
Reason (R): The peptide bond formation in prokaryotes is catalysed by a ribozyme.
 
 
1. Both (A) and (R) are True but (R) does not correctly explain (A)
2. (A) is True but (R) is False.
3. Both (A) and (R) are False.
4. Both (A) and (R) are True and (R) correctly explains (A)
Subtopic:  Transcription |
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In a transcription unit, the location of promoter is described as:
1. at the 3' end, downstream of the structural gene
2. at the 3' end, upstream of the structural gene
3. at the 5' end, upstream of the structural gene
4. at the 5' end, downstream of the structural gene
Subtopic:  Transcription |
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The ribozyme that catalyses peptide bond formation in prokaryotes is a part of:

1. the larger subunit of the bacterial ribosome
2. the smaller subunit of the bacterial ribosome
3. the 5’ UTR of the mRNA
4. the 3’ UTR of the mRNA
Subtopic:  Translation |
 63%
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