In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?
1. 5'GTATTC3' ; 3'CATAAG5'
2. 5'GATACT3' ; 3'CTATGA5'
3. 5'GAATTC3' ; 3'CTTAAG5'
4. 5'CTCAGT3' ; 3'GAGTCA5'
1. 5'GTATTC3' ; 3'CATAAG5'
2. 5'GATACT3' ; 3'CTATGA5'
3. 5'GAATTC3' ; 3'CTTAAG5'
4. 5'CTCAGT3' ; 3'GAGTCA5'
| Statement I: | Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. |
| Statement II: | Restriction endonucleases cut the DNA strand a little away from the centre of the Palindromic site. |
In the light of the above statements, choose the most appropriate answer from the options given below:
1. Statement I is incorrect but Statement II is correct.
2. Both Statement I and Statement II are correct.
3. Both Statement I and Statement II are incorrect.
4. Statement I is correct but Statement II is incorrect.
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)
| Assertion (A): | Restriction enzyme is a type of endonuclease |
| Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology, non-recombinants transformed bacteria grow on the medium containing ampicillin as well as the medium containing tetracycline. |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
1. Escherichia coli
2. Bacillus thuringiensis
3. Salmonella typhimurium
4. Agrobacterium tumefaciens
In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) only2. (c), (d) and (e) only
3. (a), (b) and (c) only
4. (a), (c), (d) and (e) only
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
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