Which of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Subtopic: Â Cloning Vector |
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Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)
| Assertion (A): | Restriction enzyme is a type of endonuclease |
| Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Â Restriction Enzymes - Main Enzymes |
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Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology, non-recombinants transformed bacteria grow on the medium containing ampicillin as well as the medium containing tetracycline. |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Â General Design of an rDNA experiment |
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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?
1. Escherichia coli
2. Bacillus thuringiensis
3. Salmonella typhimurium
4. Agrobacterium tumefaciens
Subtopic: Â Process of Biotech |Â Introduction & History |
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In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :
1. High temperature
2. Two sets of Primers
3. Taq DNA polymerase
4. Deoxynucleotides
Subtopic: Â Polymerase Chain Reaction: PCR |
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Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) only2. (c), (d) and (e) only
3. (a), (b) and (c) only
4. (a), (c), (d) and (e) only
Subtopic: Â Cloning Vector |Â Tools |
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Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
A. Isolation of foreign DNA
B. Ligation of DNA to plasmid
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A > B > C > E > D
2. A > B > E > C > D
3. A > C > B > E > D
4. A > D > E > B > C
Subtopic: Â Tools |Â General Design of an rDNA experiment |
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Which of the following DNA is used by Agrobacterium tumefaciens to transform normal cells into a tumor?
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
1. B-DNA
2. Z-DNA
3. T-DNA
4. A-DNA
Subtopic: Â Transforming Plant & Animal Cell |
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Level 1: 80%+
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Selection of recombinations based on their inability to produce colour in the presence of a chromogenic substrate is
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
1. Insertional inactivation
2. Insertional mutagenesis
3. Insertional selection
4. Insertional chromogenesis
Subtopic: Â Cloning Vector |
 89%
Level 1: 80%+
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Restriction endonucleases are bacterial enzymes that cut double stranded DNA. These restriction enzymes do not cut their own DNA because
| 1. | The bacterium does not contain the specific recognition sequence |
| 2. | The DNA is packed with histones |
| 3. | The specific recognition sequences are masked with methyl groups |
| 4. | Restriction endonucleases cut viral DNA only |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 83%
Level 1: 80%+
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