Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
1.
DNA ligase
cutting DNA, creating sticky ends of restriction fragments
2.
DNA polymerase
polymerase chain reaction to amplify sections of DNA
3.
Electrophoresis
separation of DNA fragments
4.
Reverse transcriptase
production of cDNA from mRNA
Subtopic: Â Tools |
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Level 1: 80%+
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The source of the reverse transcriptase used in recombinant DNA technology, is a:
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
1. retrovirus
2. plant cell
3. cultured fungal cell
4. cultured phage-infected mammalian cell
Subtopic: Â General Design of an rDNA experiment |
 84%
Level 1: 80%+
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Consider the two statements:
| I: | Agar Gel electrophoresis is useful in analysis of DNA. |
| II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Â Separation and Isolation of DNA fragments |
 71%
Level 2: 60%+
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Consider the given two statements:
1. Both I and II are correct and II explains I
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
| I. | It is beneficial to have a multiple cloning site (MCS) within the lac Z gene. |
| II. | When foreign DNA interrupts the lac Z gene, no b-galactosidase can be formed, and X-Gal remains colourless which allows the researcher to distinguish between recombinant and unrecombinant vectors. |
2. Both I and II are correct but II does not explain I
3. I is correct but II is incorrect
4. Both I and II are incorrect
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
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Bacterial cells synthesize restriction endonucleases because restriction endonucleases help the bacterial cells:
1. grow readily in medium containing antibodies.
2. protect themselves from viral infection.
3. open up the DNA in their chromosomes so that transcription is easier.
4. take up foreign DNA from their environment and combine it into their genome.
1. grow readily in medium containing antibodies.
2. protect themselves from viral infection.
3. open up the DNA in their chromosomes so that transcription is easier.
4. take up foreign DNA from their environment and combine it into their genome.
Subtopic: Â Introduction & History: I |Â Introduction & History: II |Â Restriction Enzymes - Main Enzymes |
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Level 2: 60%+
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Consider the following two statements:
| I: | Plasmid can be used as a vector in recombinant DNA technology experiments. |
| II: | Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Â Tools: Vectors: I |Â Cloning Vector |
 55%
Level 3: 35%-60%
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What is true regarding Downstream Processing?
| I: | This stage is after the biosynthetic stage. |
| II: | The processes include separation and purification. |
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Â Large Scale Production |
 90%
Level 1: 80%+
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Identify the correct statement:
| 1. | Agrobacterium tumefaciens is able to transform large number of monocot plants. |
| 2. | Micro-injection is the only way to transform animal cells. |
| 3. | Competence in bacterial cells can be induced by trivalent anions like phosphates. |
| 4. | Biolistics or gene gun is a suitable method to transform plant cells. |
Subtopic: Â Transforming Plant & Animal Cell |
 83%
Level 1: 80%+
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What controls the copy number of linked DNA in a transformed bacterial cell?
| 1. | Ori | 2. | Cloning sites |
| 3. | Selectable marker | 4. | Reporter gene |
Subtopic: Â Cloning Vector |
 89%
Level 1: 80%+
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The fragments created by cutting DNA with restriction endonucleases can be separated by gel electrophoresis. What is incorrect regarding this procedure?
| 1. | The fragments move towards anode. |
| 2. | Agar gel is used for sieving effect. |
| 3. | DNA fragments can be visualized moving in the medium if stained with ethidium bromide followed by exposure to UV radiation. |
| 4. | The larger fragments move farther in the medium. |
Subtopic: Â Separation and Isolation of DNA fragments |
 87%
Level 1: 80%+
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