Bacterial cells synthesize restriction endonucleases because restriction endonucleases help the bacterial cells:
1. grow readily in medium containing antibodies.
2. protect themselves from viral infection.
3. open up the DNA in their chromosomes so that transcription is easier.
4. take up foreign DNA from their environment and combine it into their genome.
Subtopic: Â Introduction & History: I |Â Introduction & History: II |Â Restriction Enzymes - Main Enzymes |
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Consider the following two statements:
| I: | Plasmid can be used as a vector in recombinant DNA technology experiments. |
| II: | Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria. |
| 1. | Both I and II are correct and II explains I |
| 2. | Both I and II are correct but II does not explain I |
| 3. | I is correct but II is incorrect |
| 4. | Both I and II are incorrect |
Subtopic: Â Tools: Vectors: I |Â Cloning Vector |
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What is true regarding Downstream Processing?
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
I. This stage is after the biosynthetic stage.
II. The processes include separation and purification.
| 1. | Only I | 2. | Only II |
| 3. | Both I and II | 4. | Neither I nor II |
Subtopic: Â Large Scale Production |
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Identify the correct statement:
| 1. | Agrobacterium tumefaciens is able to transform large number of monocot plants. |
| 2. | Micro-injection is the only way to transform animal cells. |
| 3. | Competence in bacterial cells can be induced by trivalent anions like phosphates. |
| 4. | Biolistics or gene gun is a suitable method to transform plant cells. |
Subtopic: Â Transforming Plant & Animal Cell |
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What controls the copy number of linked DNA in a transformed bacterial cell?
| 1. | Ori | 2. | Cloning sites |
| 3. | Selectable marker | 4. | Reporter gene |
Subtopic: Â Cloning Vector |
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The fragments created by cutting DNA with restriction endonucleases can be separated by gel electrophoresis. What is incorrect regarding this procedure?
| 1. | The fragments move towards anode. |
| 2. | Agar gel is used for sieving effect. |
| 3. | DNA fragments can be visualized moving in the medium if stained with ethidium bromide followed by exposure to UV radiation. |
| 4. | The larger fragments move farther in the medium. |
Subtopic: Â Separation and Isolation of DNA fragments |
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The discipline of biotechnology was founded on the breakthrough work of:
| 1. | Bolivar and Rodriquez | 2. | Cohen and Boyer |
| 3. | Nathans and Daniels | 4. | Redi and Spallanzani |
Subtopic: Â Introduction & History: I |Â Introduction & History: II |Â Restriction Enzymes: Historical Background |
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In 1970, Hamilton O. Smith, Thomas Kelly and Kent Wilcox isolated and characterized the first type II restriction enzyme that was:
| 1. | EcoR I | 2. | Hind II |
| 3. | Bam HI | 4. | Sma I |
Subtopic: Â Restriction Enzymes - Main Enzymes |
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In a continuous culture system:
| I: | The used medium is drained out from one side while the fresh medium is added from the other. |
| II: | The cells are maintained in their physiologically most active log/exponential phase. |
| III: | A larger biomass is produced leading to higher yields of desired proteins. |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II and III are correct |
Subtopic: Â Large Scale Production |
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A plasmid contains two genes for resistance to separate antibiotics. One of the two is insertionally inactivated due to insertion of alien DNA.
| I: | The gene that was not inactivated helps in selecting the transformants. |
| II: | The gene that was insertionally inactivated helps in selection of recombinants. |
| 1. | Only I is correct |
| 2. | Only II is correct |
| 3. | Both I and II are correct |
| 4. | I, II and III are correct |
Subtopic: Â Cloning Vector |Â Selection of Recombinant Transformants |
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