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Q. No.'Heat shock' method in bacterial transformation:
1.
allows selection of transformants
2.
promotes uptake of DNA through membrane transport proteins
3.
creates pores in the bacterial plasma membrane and allows plasmid
DNA to enter the bacterial DNA.
4.
coagulates DNA in the bacterial cell.
DNA to enter the bacterial DNA.
Subtopic: Transforming Plant & Animal Cell |
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Level 2: 60%+
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of phosphodiester bond in DNA by the enzyme |
| 2. | Cutting of DNA at specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Subtopic: Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
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The process by which a virus transfers genetic material from one bacterium to another is called:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
Subtopic: Tools |
51%
Level 3: 35%-60%
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Enzymes that catalyze the removal of nucleotides in either the 5-prime to 3-prime or the 3-prime to 5-prime direction from the ends of single-stranded and/or double-stranded DNA are called:
| 1. | endonuclease | 2. | exonuclease |
| 3. | ligase | 4. | polymerase |
Subtopic: Introduction & History: I | Introduction & History: II |
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Level 2: 60%+
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Consider the given two statements
| Assertion (A): | In agar gel electrophoresis, smaller DNA fragments move farther than the larger DNA fragments. |
| Reason (R): | In agar gel electrophoresis, DNA fragments separate according to their size due to the sieving effect of the agarose gel. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True, (R) is False. |
| 4. | (A) is False, (R) is False. |
Subtopic: Separation and Isolation of DNA fragments |
88%
Level 1: 80%+
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Plasmids
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I: | are inherited from one generation to the next. |
| II: | may carry genes that give their host a selective advantage. |
| III: | can render bacteria drug-resistant. |
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Cloning Vector |
80%
Level 1: 80%+
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Consider the two statements:
| Assertion (A): | Restriction enzymes produced by bacterial cells do not harm their own DNA |
| Reason (R): | Bacterial DNA are single-stranded molecules. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True, (R) is False |
| 4. | (A) is False, (R) is True |
Subtopic: Restriction Enzymes - Main Enzymes |
79%
Level 2: 60%+
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Consider the given two statements:
| I: | Transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). |
| II: | Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another. |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Tools |
67%
Level 2: 60%+
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The origin of replication sequence in a plasmid:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | is a sequence from where replication starts |
| II: | is a sequence responsible for controlling the copy number of linked DNA |
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Cloning Vector |
95%
Level 1: 80%+
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Consider the given two statements:
| Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
| Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | Both (A) and (R) are False. |
| 4. | (A) is True, (R) is False. |
Subtopic: Restriction Enzymes - Main Enzymes |
56%
Level 3: 35%-60%
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