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Q. No.Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule
\(5'C-T-G-C-A \downarrow G3\\ 3'G-A-C-G-T \downarrow C5'\\ ~~~~~~\uparrow\)
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I
1.
\(5'C-T-G~\\ 3'-G-A-C-G-T'\)
\(C-A-G3'\\ C'5\)
2.
\(5'C-T\\ 3'G-A-G-C \)
\(G-C-A-G3'\\ T-C'5\)
3.
\(5'C-T-G-C \\ 3'G-A-C-G \)
\(A-G3'\\ T'-C5'\)
4.
\(5'C-T-G-C-A\\ 3'G\)
\(3'G\\ A-C-G-T-C'5\)
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 3: 35%-60%
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Assertion(A): Synthetic oligonucleotide polymers are used during Annealing in a PCR
Reason(R): The primers bind to the double stranded DNA at their complementary regions
Reason(R): The primers bind to the double stranded DNA at their complementary regions
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are true, but (R) is not the correct explanation of (A) |
| 3. | (A) is true, but (R) is false |
| 4. | (A) is false, but (R) is true |
Subtopic: Polymerase Chain Reaction: PCR |
Level 3: 35%-60%
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Match the process of PCR in column I with the corresponding temperature in column II and select the correct option
1. (a)-(ii), (b)-(i), (c)-(iii)
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
| Column-I | Column-II | ||
| (a) | Heat denaturation | (i) | 70-75°C |
| (b) | Annealing | (ii) | 90-98°C |
| (c) | Primer extension | (iii) | 40-60°C |
2. (a)-(i), (b)-(iii), (c)-(ii)
3. (a)-(i), (b)-(ii), (c)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(i)
Subtopic: Polymerase Chain Reaction: PCR |
64%
Level 2: 60%+
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Consider the given two statements:
| Assertion (A): | A piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. |
| Reason (R): | The vectors provide the origin of replication. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is False but (R) is True. |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: Tools |
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Level 2: 60%+
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Introduction of an alien DNA into plant host cell is achieved by making them
1. Competent with bivalent ions
2. Using microinjections
3. Using gene gun
4. Using lysozymes and chitinase
1. Competent with bivalent ions
2. Using microinjections
3. Using gene gun
4. Using lysozymes and chitinase
Subtopic: Transforming Plant & Animal Cell |
62%
Level 2: 60%+
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Subtopic: Process of Biotech |
91%
Level 1: 80%+
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Which of the following does not hold true for restriction enzymes?
| 1. | It recognises a palindromic nucleotide sequence. |
| 2. | It is an endonuclease. |
| 3. | It is isolated from viruses. |
| 4. | it can produce the same kind of sticky ends in different DNA molecules. |
Subtopic: Restriction Enzymes - Main Enzymes |
80%
Level 1: 80%+
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Name the enzymes 'P' and 'Q' that are involved in the processes given below.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
1. Enzyme P-Exonuclease and Enzyme Q-permease.
2. Enzyme P-Exonuclease and Enzyme Q-Ligase.
3. Enzyme P-Endonuclease and Enzyme Q-permease.
4. Enzyme P-Restriction endonuclease and Enzyme Q-Ligase.
Subtopic: Restriction Enzymes - Main Enzymes |
80%
Level 1: 80%+
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| Assertion (A): | During the heat shock method, the cells are incubated with recombinant DNA at \(42^{\circ} \mathrm{C}\), following by placing them briefly on ice and then putting if back at \(42^{\circ} \mathrm{C}\). |
| Reason (R): | After heat shock, uptake of DNA through transient pores in the bacterial cell wall takes place. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true but (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | (A) is false but (R) is true. |
Subtopic: Transforming Plant & Animal Cell |
Level 3: 35%-60%
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If an enzyme catalyzes the removal of nucleotides from the ends of DNA, it will be called as a/an:
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
1. endonuclease
2. exonuclease
3. DNA ligase
4. DNA polymerase
Subtopic: Restriction Enzymes - Main Enzymes |
90%
Level 1: 80%+
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