In annealing:
1. Primers anneal to the DNA template
2. Taq polymerase adds nucleotides to the annealed primer
3. Two strands of the DNA separate
4. Temperature in 92° C

Subtopic:  Polymerase Chain Reaction: PCR |
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Level 2: 60%+
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In Annealing:
1. Primers anneal to the DNA template
2. Taq polymerase adds nucleotides to the annealed primer
3. Two strands of the DNA separate
4. Temperature is 92ºC

 
Subtopic:  Polymerase Chain Reaction: PCR |
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Level 1: 80%+
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Which is the first step in PCR cycle?
1. Denaturation
2. Extension
3. Annealing
4. None
Subtopic:  Polymerase Chain Reaction: PCR |
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Level 1: 80%+
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Which is also called molecular glue:
1. DNA Gyrase
2. DNA Helicase
3. DNA Ligase
4. DNA Polymerase
Subtopic:  Tools |
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Level 1: 80%+
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Gene transfer is present in:
1. biolistics
2. hybridisation
3. tissue culture
4. vegetative propagation
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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All monerans:
1. contain DNA and RNA
2. demonstrate a long circular strand of DNA, not formed enclosed in a nuclear membrane
3. are bacteria
4. All of the above

 
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Which of the following is used as a best genetic vector in plants?
1. Bacillus thurengiensis
2. Agrobacterium tumifaciens
3. Pseudomonas putida
4. All of the above 
Subtopic:  Tools |
 75%
Level 2: 60%+
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Which is also called molecular glue?
1. DNA gyrase
2. DNA helicase
3. DNA ligase
4. DNA polymerase
Subtopic:  Tools |
 95%
Level 1: 80%+
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Genetic variation cannot be effectively brought about by?
1. Transduction
2. Mutation
3. Transformation
4. Amplification
Subtopic:  Polymerase Chain Reaction: PCR |
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Level 2: 60%+
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Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule 
\(5'C-T-G-C-A \downarrow G3\\ 3'G-A-C-G-T \downarrow C5'\\ ~~~~~~\uparrow\)
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I 
1. \(5'C-T-G~\\ 3'-G-A-C-G-T'\) \(C-A-G3'\\ C'5\)
2.  \(5'C-T\\ 3'G-A-G-C \) \(G-C-A-G3'\\ T-C'5\)
3.  \(5'C-T-G-C \\ 3'G-A-C-G \) \(A-G3'\\ T'-C5'\)
4. \(5'C-T-G-C-A\\ 3'G\) \(3'G\\ A-C-G-T-C'5\)
Subtopic:  Restriction Enzymes - Main Enzymes |
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