Complete the analogy and select the correct option.
BamHI : Sticky ends : : EcoRV : ______
1. Staggered ends
2. Cohesive ends
3. Blunt ends
4. Ends with overhanging stretches
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 2: 60%+
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All of the given statements are true with respect to the separation of DNA fragments by gel electrophoresis except:
| 1. | The cutting of DNA by restriction endonucleases results in fragments of DNA and these fragments can be separated by this technique. |
| 2. | The most commonly used matrix is agarose which is a natural polymer extracted from seaweeds. |
| 3. | The DNA fragments resolve according to their size. Hence, the larger the fragment size, the farther it moves. |
| 4. | The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide. |
Subtopic: Separation and Isolation of DNA fragments |
88%
Level 1: 80%+
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EcoRI cuts the DNA between bases _______ and ______ only when the recognition sequence GAATTC is present in the DNA.
Select the correct option to fill the blanks respectively.
1. A and T
2. T and C
3. G and A
4. A and C
Select the correct option to fill the blanks respectively.
1. A and T
2. T and C
3. G and A
4. A and C
Subtopic: Tools: Enzymes: I | Restriction Enzymes - Main Enzymes |
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Level 2: 60%+
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How many cycles of PCR are required for obtaining 256 molecules of dsDNA, if your starting material was one molecule of dsDNA?
1. 4
2. 9
3. 128
4. 8
1. 4
2. 9
3. 128
4. 8
Subtopic: Polymerase Chain Reaction: PCR |
61%
Level 2: 60%+
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Select the incorrect match with respect to rDNA technology:
| 1. | Bioprocess engineering | – | Maintenance of sterile ambience |
| 2. | Genetic engineering | – | Alteration of genetic material |
| 3. | Downstream processing | – | Biosynthetic stage |
| 4. | Biosynthetic stage | – | Culturing of recombinant host cells |
Subtopic: Bioreactors/Downstream Processing |
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Level 2: 60%+
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Match column I with column II and choose the correct option:
1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
| Column I | Column II | ||
| a. | Salmonella typhimurium | (i) | Source of EcoRI |
| b. | Bacillus thuringiensis | (ii) | Used in developing transgenic tobacco plant |
| c. | Agrobacterium tumefaciens |
(iii) | Used in creating first recombinant DNA |
| d. | Escherichia coli | (iv) | Gene used in creating biopesticide |
1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic: Restriction Enzymes - Main Enzymes | Transforming Plant & Animal Cell | Restriction Enzymes: Historical Background |
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Level 1: 80%+
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During the process of isolation of DNA, chilled ethanol is added to:
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
Subtopic: General Design of an rDNA experiment |
87%
Level 1: 80%+
NEET - 2013
Hints
| Assertion (A): | Inserting alien DNA in pBR322 plasmid at Pst I makes the transformants sensitive to ampicillin antibiotic. |
| Reason (R): | Restrictions site for Pst I is present on ampR . |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true, but (R) is not the correct explanation of (A). |
| 3. | (A) is true, but (R) is false. |
| 4. | Both (A) and (R) are false. |
Subtopic: Tools: Vectors: I | Tools |
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| Assertion (A): | DNA ligase plays important role in recombinant DNA technology. |
| Reason (R): | The linking of antibiotic resistant gene with plasmid vector became possible by enzyme DNA ligase. |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are true, but (R) is not the correct explanation of (A). |
| 3. | (A) is true, but (R) is false. |
| 4. | Both (A) and (R) are false. |
Subtopic: General Design of an rDNA experiment |
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Level 1: 80%+
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Assertion (A): An alien DNA is linked with the origin of replication for making multiple identical copies.
Reason (R): An origin of replication is a specific DNA sequence which is responsible for initiating replication
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Reason (R): An origin of replication is a specific DNA sequence which is responsible for initiating replication
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true, but (R) is not the correct explanation of (A).
3. (A) is true, but (R) is false.
4. Both (A) and (R) are false.
Subtopic: General Design of an rDNA experiment |
84%
Level 1: 80%+
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