In agar gel electrophoresis, the restriction fragments produced by restriction enzymes:
1.
move towards cathode
2.
do not move at all
3.
are separated according to size with smaller fragments moving farther
4.
are transported onto a nitrocellulose membrane
Subtopic: Separation and Isolation of DNA fragments |
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Level 1: 80%+
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DNA can be visualized through UV rays if it is stained with:
| 1. | Ethidium bromide | 2. | Polyethylene glycol |
| 3. | Tritiated thymidine | 4. | Colchicine |
Subtopic: Separation and Isolation of DNA fragments |
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The Ti Plasmid [ having T-DNA] is considered a natural genetic engineer and can transform:
1. bacterial cells
2. dicot plant cells
3. monocot plant cells
4. animal cells
1. bacterial cells
2. dicot plant cells
3. monocot plant cells
4. animal cells
Subtopic: Cloning Vector |
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Normally, microinjection and biolistics [gene gun] are used, respectively, to transform:
1. plant cells and animal cells
2. animal cells and plant cells
3. animal cells and bacterial cells
4. plant cells and bacterial cells
1. plant cells and animal cells
2. animal cells and plant cells
3. animal cells and bacterial cells
4. plant cells and bacterial cells
Subtopic: Transforming Plant & Animal Cell |
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It is usually necessary to induce ‘competence’ in bacterial cells to transform them in recombinant DNA procedures mainly because:
| 1. | bacterial cell has a cell wall |
| 2. | bacteria do not have a well defined nucleus and membrane bound organelles |
| 3. | DNA is a hydrophilic molecule |
| 4. | DNA is associated with positively charged histone proteins |
Subtopic: Tools |
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It is possible to make human proteins in bacterial cells with rDNA experiments because:
| 1. | bacterial cells replicate faster than eukaryotic cells |
| 2. | genetic code is almost universal |
| 3. | bacterial cells have plasmids that can be used as a vector |
| 4. | bacterial cells produce restriction enzymes |
Subtopic: General Design of an rDNA experiment |
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Polymerase chain reaction can amplify DNA, producing about 1 billion copies in 30 cycles. The correct sequence of the steps of this reaction is:
| 1. | Denaturation → Annealing of primers → Extension of primers |
| 2. | Denaturation → Extension of primers → Annealing of primers |
| 3. | Extension of primers → Annealing of primers → Denaturation |
| 4. | Annealing of primers → Extension of primers → Denaturation |
Subtopic: Polymerase Chain Reaction: PCR |
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Level 1: 80%+
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Taq polymerase is:
| 1. | is a ribozyme involved in RNA splicing in eukaryotes |
| 2. | is a thermostable DNA polymerase used in PCR |
| 3. | is a key reagent in ELISA |
| 4. | an enzyme isolated from Thermus aquaticus, a fungus found in damp soils |
Subtopic: Polymerase Chain Reaction: PCR |
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Level 1: 80%+
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Identify the incorrect statement:
| 1. | The process of separation and purification of expressed protein before marketing is called as downstream processing |
| 2. | DNA precipitation out of a mixture of biomolecules can be achieved by treatment with a divalent cation such as calcium ion |
| 3. | Stirred-tank bioreactors have been designed for availability of oxygen throughout the process |
| 4. | Reporter genes can be used as markers for selecting successfully transformed cells |
Subtopic: Process of Biotech |
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The first restriction endonuclease, whose functioning depended on a specific DNA nucleotide sequence was:
| 1. | EcoR I | 2. | BamH I |
| 3. | Hind II | 4. | Sma I |
Subtopic: Restriction Enzymes - Main Enzymes |
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