It is desirable to use restriction enzymes that create sticky ends in recombinant DNA technology procedures because this:
1.
facilitates the action of DNA ligase
2.
makes selection of recombinant DNA possible
3.
increases the copy number of rDNA per cell
4.
will create multiple fragments of the vector DNA
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II | Tools |
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Plasmids are good cloning vectors mainly because:
| 1. | they are circular |
| 2. | they replicate autonomously |
| 3. | they are extra-chromosomal DNA molecules found in many bacterial cells |
| 4. | they carry genes vital for normal survival and reproduction |
Subtopic: Tools: Vectors: I |
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In a plasmid such as pBR322:
| I: | Ori controls the copy number of DNA per cell |
| II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
| III: | Genes for antibiotic resistance are used as selectable markers |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II, and III are correct |
Subtopic: Cloning Vector |
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A plasmid has genes for resistance against ampicillin and tetracycline. The tetracycline resistant gene is insertionally inactivated by inserting a foreign DNA within the gene. The bacterial cells are transformed. The recombinant transformants:
| 1. | will be killed in a medium containing ampicillin but not in a medium containing tetracycline |
| 2. | will be killed in a medium containing tetracycline but not in a medium containing ampicillin |
| 3. | will be killed in a medium containing ampicillin and in a medium containing tetracycline |
| 4. | will not be killed in a medium containing ampicillin and in a medium containing tetracycline |
Subtopic: Cloning Vector |
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In agar gel electrophoresis, the restriction fragments produced by restriction enzymes:
| 1. | move towards cathode |
| 2. | do not move at all |
| 3. | are separated according to size with smaller fragments moving farther |
| 4. | are transported onto a nitrocellulose membrane |
Subtopic: Separation and Isolation of DNA fragments |
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DNA can be visualized through UV rays if it is stained with:
| 1. | Ethidium bromide | 2. | Polyethylene glycol |
| 3. | Tritiated thymidine | 4. | Colchicine |
Subtopic: Separation and Isolation of DNA fragments |
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The Ti Plasmid [ having T-DNA] is considered a natural genetic engineer and can transform:
1. bacterial cells
2. dicot plant cells
3. monocot plant cells
4. animal cells
1. bacterial cells
2. dicot plant cells
3. monocot plant cells
4. animal cells
Subtopic: Cloning Vector |
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Normally, microinjection and biolistics [gene gun] are used, respectively, to transform:
1. plant cells and animal cells
2. animal cells and plant cells
3. animal cells and bacterial cells
4. plant cells and bacterial cells
1. plant cells and animal cells
2. animal cells and plant cells
3. animal cells and bacterial cells
4. plant cells and bacterial cells
Subtopic: Transforming Plant & Animal Cell |
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It is usually necessary to induce ‘competence’ in bacterial cells to transform them in recombinant DNA procedures mainly because:
| 1. | bacterial cell has a cell wall |
| 2. | bacteria do not have a well defined nucleus and membrane bound organelles |
| 3. | DNA is a hydrophilic molecule |
| 4. | DNA is associated with positively charged histone proteins |
Subtopic: Tools |
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It is possible to make human proteins in bacterial cells with rDNA experiments because:
| 1. | bacterial cells replicate faster than eukaryotic cells |
| 2. | genetic code is almost universal |
| 3. | bacterial cells have plasmids that can be used as a vector |
| 4. | bacterial cells produce restriction enzymes |
Subtopic: General Design of an rDNA experiment |
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