A circular plasmid of 10000 bp is digested with two restriction enzymes A and B, to produce 3000 bp and 2000 bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A [A1] is present at the 100th base, the order in which the remaining sites [A2, B1 and B2] are present is:
1. 5100, 3100, 8100 2. 3100, 5100, 8100 3. 8100, 5100, 3100 4. 8100, 3100, 5100
| 1. | Ori site | 2. | Palindromic sequence |
| 3. | Recognition site | 4. | Selectable marker |
In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
Identify the wrong statement with regards to restriction enzyme:
| 1. | They cut the strand at palindromic sites. |
| 2. | They are useful in genetic engineering. |
| 3. | Sticky ends can be joined by using DNA ligases. |
| 4. | Each restriction enzyme functions by inspecting the length of a DNA sequence. |
The specific palindromic sequence which is recognized by EcoRI is:
| 1. | 5' -GGAACC-3' 3'-CCTTGG-5' |
2. | 5'-CTTAAG-3' 3'-GAATTC-5' |
| 3. | 5'-GGATCC-3' 3'-CCTAGG-5' |
4. | 5'-GAATTC-3' 3'-CTTAAG-5' |
The downstream processing stage of rDNA does not involve:
| 1. | Biosynthesis | 2. | Separation |
| 3. | Purification | 4. | Preservation |
The enzyme used for DNA amplification in PCR procedures is:
1. Polynucleotide phosphorylase
2. Reverse transcriptase
3. Taq polymerase
4. DNA-dependent RNA polymerase
To isolate DNA in pure form from a bacterial cell, it should initially be treated with:
| 1. | Proteases and RNase but not DNase | 2. | Lysozyme |
| 3. | Restriction endonuclease | 4. | DNA ligase |
In microinjection, the rDNA :
| 1. | coats the micropellets and is used in biolistics to transform plant cells |
| 2. | is directly injected into the cytoplasm of a plant or an animal cell |
| 3. | is delivered in the vicinity of the plasma membrane of the cell (to be transformed) |
| 4. | is directly injected into the nucleus of an animal cell |
Which of the following can be used to induce competence for getting a bacterial cell transformed during rDNA procedures?
| 1. | A divalent cation | 2. | Ethidium bromide |
| 3. | Polyethylene glycol | 4. | Anionic proteins |
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