If a researcher start his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

A.      Cellulase

B.      Protease

C.      Ribonuclease

D.     Chitinase

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
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If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

A.      Cellulase

B.      Chitinase

C.      Lysozyme

D.     Protease

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 86%
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If a researcher has to use a bacterial cell to isolate DNA from bacterial cell then to remove other nucleic acid, which enzyme should be used?

A.      DNAse

B.      Ribonuclease

C.      Protease

D.     Lysozyme

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 62%
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The precipitation of DNA out of the solution is done by adding

A.      Organic Acid

B.      HCl

C.      Ethanol

D.     Ketone

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
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The end result of precipitation of DNA in suspension is seen

A.      In the form of dark solution

B.      In the form of effervescence

C.      In the form of fine threads

D.     In the form of bubbles

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
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The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
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The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

Subtopic:  Large Scale Production: I | Large Scale Production |
 59%
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The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Subtopic:  Large Scale Production: I | Large Scale Production |
 54%
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Which of the following doesn't go with PCR?

A.      Thermus aquaticus

B.      1 billion copies

C.      Taq polymerase

D.     Dideoxynucleotides

Subtopic:  Large Scale Production: I | Large Scale Production |
 89%
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What is the final step of PCR?

A.      Extension

B.      Annealing

C.      Denaturation

D.     Extraction

Subtopic:  Large Scale Production: I | Large Scale Production |
 85%
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