Elution is:

1. Separating the restricted DNA fragments on agarose gel.

2. Staining the separate DNA fragments with ethidium bromide

3. cutting out of the separated band of DNA from the agarose gel and extracting them from the gel piece.

4. constructing rDNA by joining the purified DNA fragments to the cloning vector.

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 78%
From NCERT
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The first type II restriction endonuclease discovered that could cut dsDNA specific site was:

1. EcoRI

2. SmaI

3. Hind II

4. Hind III

Subtopic:  Tools: Enzymes: II | Tools |
 90%
From NCERT
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Which statement regarding restriction endonucleases is NOT correct?

1. They recognize a specific base sequence in the DNA.

2. They are produced by bacterial cells as a primitive immune system.

3. They digest DNA by removing nucleotides from a free 3' end.

4. They often generate stick ends.

Subtopic:  Tools: Enzymes: II | Tools |
 76%
From NCERT
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Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?

1. BamHI

2. Psti

3. Clal

4. Pvull

Subtopic:  Tools: Vectors: II | Tools |
 92%
From NCERT
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In the screening process during rDNA experiments, clones that metabolize β-gal turn:

1. Colorless

2. Blue

3. Yellow

4. Green

Subtopic:  Selectable Markers: III | Selectable Markers |
 70%
From NCERT
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Microinjection is suitable for :

1. Injecting an ovum into the sperm in IVF

2. Transforming animal cells

3. Injecting very small sized drug particles into neurons

4. Conferring antibiotic resistance to certain strain of bacteria 

Subtopic:  Tools: Vectors: II | Tools |
 75%
From NCERT
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Polymerase Chain Reaction is not used in :

1. Confirming presence of a pathogen during early infection

2. Identifying the mutated genes in suspected cancer patients

3. Isolating the gene of interest from host DNA to be cloned by recombinant procedures

4. Detection of the presence of HIV in suspected AIDS patient

Subtopic:  Large Scale Production: I | Large Scale Production |
 72%
From NCERT
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The plasmid pBR322 does not contain:

1. An origin of replication site

2. A gene that encodes for restrictor of plasmid copy number

3. Gene for Ampicillin and Streptomycin resistance

4. Sites for many restriction enzymes

Subtopic:  Tools: Vectors: II | Tools |
 71%
From NCERT
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Restriction enzymes are synthesized by:

1. Bacteria only

2. Yeast and bacteria only

3. Eukaryotic cells only

4. All kinds of cells

Subtopic:  Restriction Enzymes: Historical Background |
 54%
From NCERT
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The bioreactor shown in the diagram is:

1. Sparged stirred tank type

2. Airlift type

3. Simple stirred tank type

4. Fluidized bed type

Subtopic:  Large Scale Production: II | Large Scale Production |
 78%
From NCERT
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