In order to make the host cell competent

Divalent cations such as calcium are used-

A.      The divalent ions has to be in specific concentration

B.      They cause the DNA uptake by the cell

C.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

D.     Both B and C

Subtopic:  Host & Desired DNA: I | Host & Desired DNA |
 83%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

rop segment codes for pBR322?

1. Resistance protein

2. Protein for replication

3. Proteins for translation

4. Cloning site

Subtopic:  Cloning Vector |
 74%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

If a researcher start his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

A.      Cellulase

B.      Protease

C.      Ribonuclease

D.     Chitinase

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 94%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

advertisementadvertisement

If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

A.      Cellulase

B.      Chitinase

C.      Lysozyme

D.     Protease

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 86%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

If a researcher has to use a bacterial cell to isolate DNA from bacterial cell then to remove other nucleic acid, which enzyme should be used?

A.      DNAse

B.      Ribonuclease

C.      Protease

D.     Lysozyme

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 62%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

The precipitation of DNA out of the solution is done by adding

A.      Organic Acid

B.      HCl

C.      Ethanol

D.     Ketone

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 97%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

advertisementadvertisement

The end result of precipitation of DNA in suspension is seen

A.      In the form of dark solution

B.      In the form of effervescence

C.      In the form of fine threads

D.     In the form of bubbles

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

The precipitated DNA can be removed from the suspension

A.      By Filtration

B.      By giving a heat shock

C.      By spooling

D.     By gel electrophoresis

Subtopic:  Host & Desired DNA: II | Host & Desired DNA |
 88%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

Subtopic:  Large Scale Production: I | Large Scale Production |
 59%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch

advertisementadvertisement

The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Subtopic:  Large Scale Production: I | Large Scale Production |
 54%
From NCERT
To view explanation, please take trial in the course.
NEET 2025 - Target Batch
Hints
To view explanation, please take trial in the course.
NEET 2025 - Target Batch