Which of the following step is first one in order to separate DNA fragments?

A.      Running of gel

B.      Staining of gel

C.      Elution

D.     Setting of gel

In a DNA gel the fragment of 2kb and 3kb will be

1. 2kb nearer to cathode while 3kb nearer to anode

2. 2kb closer to wells while 3kb towards the opposite ends

3. 2kb closer to opposite end of gel while 3kb closer to well

4. 2kb away from cathode while 3kb towards anode

If I want to check the expression of my gene, then I should use

A.      Vectors supporting high copy number

B.      Vectors supporting low copy number

C.      No need to bother about copy number

D.     Vectors with strong Ori

Agrobacterium tumifaciens

1. A pathogen of  angiosperms

2. It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

3. Ti plasmid of it is now modified into an expression vector

4. It's Ti plasmid is modified into a cloning vector which is no more pathogenic

In order to make the host cell competent

Divalent cations such as calcium are used-

A.      The divalent ions has to be in specific concentration

B.      They cause the DNA uptake by the cell

C.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

D.     Both B and C

rop segment codes for pBR322?

1. Resistance protein

2. Protein for replication

3. Proteins for translation

4. Cloning site

If a researcher start his work of DNA extraction from a plant cell, the very first enzyme, he would use will be

A.      Cellulase

B.      Protease

C.      Ribonuclease

D.     Chitinase

If a researcher has to use a bacterial cell to work with after cell wall breaking which enzyme he needs to use?

A.      Cellulase

B.      Chitinase

C.      Lysozyme

D.     Protease

If a researcher has to use a bacterial cell to isolate DNA from bacterial cell then to remove other nucleic acid, which enzyme should be used?

A.      DNAse

B.      Ribonuclease

C.      Protease

D.     Lysozyme

The precipitation of DNA out of the solution is done by adding

A.      Organic Acid

B.      HCl

C.      Ethanol

D.     Ketone