A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :
1. ampicillin but not tetracycline
2. tetracycline but not ampicillin
3. both tetracycline and ampicillin
4. neither tetracycline nor ampicillin
How are transformants selected from nontransformants?
1. | Presence of more than one recognition site in the vector DNA. |
2. | Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker. |
3. | Antibiotic resistance gene gets inactivated due to insertion of alien DNA. |
4. | Both 2 and 3. |