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#3 | Tools: Enzymes: I
(Zoology) > Biotechnology: Principles and Processes

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Related Practice Questions :

What is true A,B and C in the given diagrammatic representation of rDNA technology ?


 

I: At A same restriction enzyme is used to cut both foreign and vector DNA
II: The enzyme used at B is DNA ligase
III: Step C can be called as transformation

1. I and II only
2. I and III only
3. II and III only
4. I,II and III
 87%
Level 1: 80%+
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In 1960’s two enzymes were discovered in bacteria that were responsible for providing immunity against bacteriophages. One was Restriction Endonuclease and the other was

1. Methylase

2. Exonuclease

3. Aminotransferase

4. Terminal Transferase

 71%
Level 2: 60%+
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The names associated with construction of first Recombinant DNA are

1. Arber, Nathans, Smith
2. Annie Chang, Boyer, Berg, Cohen
3. Howard Temin, Brenner, Sharp
4. Tim Hunt, Hartwell, Nurse
 74%
Level 2: 60%+
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Restriction enzymes do not act on the the DNA of the Host cell in which they are produced because

1. Host DNA is packed into chromosomes
2. Restriction enzymes are ineffective on host DNA
3. Host DNA does not have the restriction site for the Restriction enzymes.
4. Restriction site of host DNA is methylated.
 56%
Level 3: 35%-60%
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Arrange the following enzymes/ processes in the order of their use for a certain experiment of genetic engineering:

1) ligase,
2) restriction enzyme
3)desired gene
4) Transformation
5) Expression

1.      3-2-1-4-5

2.      1-2-4-5-3

3.      3-1-4-5-2

4.     2-5-4-3-1

 85%
Level 1: 80%+
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