The enzyme used in the polymerase chain reaction is a :

(1) DNA dependent RNA polymerase

(2) RNA dependent DNA polymerase

(3) DNA dependent DNA polymerase

(4) RNA dependent RNA polymerase

Taq polymerase is used in the polymerase chain reaction because:

1. It replicates DNA faster than other enzymes

2. It is the only enzyme that can replicate DNA invitro

3. It does not require an RNA primer to function

4. It is thermostable 

What is true about the steps of PCR shown in the diagram given below?

l. Step A occurs at the temperature of 95-98${}^{\mathrm{o}}$ C

ll. Step B occurs at the temperature of 55${}^{\mathrm{o}}$ C

lll. Step C occurs at the temperature of 72${}^{\mathrm{o}}$ C

1. l and ll only

2. l and lll only

3. ll and lll only

4. l, ll and lll

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperature

Select the correct order of processing of PCR.

(1) Extension, primer annealing, denaturation

(2) Denaturation, primer annealing, extension

(3) Denaturation, extension, primer annealing

(4) Primer annealing, denaturation, extension

The source of taq polymerase used in PCR is a

(1) thermophilic fungus

(2) mesophilic fungus

(3) thermophilic bacterium

(4) halophilic bacterium

Primers are not

1.      Small

2.      Biologically synthesized

3.      Oligonucleotides

4.     Complementary to the regions of DNA

Which of the following doesn't go with PCR?

1.    Thermus aquaticus

2.     1 billion copies

3.     Taq polymerase

4.     Dideoxynucleotides

If a strand has the following sequence 5’ATTGCCCTAG 3’what will be the sequence of DNA synthesized from its complementary strand.

1.      5’TAACGGGATC 3’

2.      3’TAACGGGATC 5’

3.      5’ATTGCCCTAG 3’

4.     3’ATTGCCCTAG 5’

If you start with 10 molecules of Double stranded DNA, what will be the number of DNA molecules formed after 10 cycles of PCR?

1.      10240

2.      1024

3.      512

4.     5120