What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

1. The larger the fragment size, the farther it moves
2. The smaller the fragment size, the farther it moves
3. Positively charged fragments move to farther end
​​​​​​​
4. Negatively charged fragments do not move

Subtopic:  Process of Biotech |
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The DNA fragments separated on an agarose gel can be visualised after staining with

1. bromophenol blue

2. acetocarmine
3. aniline blue 

4. ethidium bromide

Subtopic:  Process of Biotech |
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

1. centrifugation
2. polymerase chain reaction
3. electrophoresis
4. restriction mapping
Subtopic:  Process of Biotech |
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Agarose extracted from sea weeds finds use in

1. tissue culture

2. PCR

3. gel electrophoresis

4. spectrophotometry

Subtopic:  Process of Biotech |
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Gel electrophoresis is used for

1. cutting of DNA into fragments

2. separation of DNA fragments according to their size

3. construction of recombinant DNA by joining with cloning vectors

4. isolation of DNA molecule

Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
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A mixture containing DNA fragments, a, b, c and d, with molecular weights of a+b = c, a > b and d>c, was subjected to agarose gel electrophoresis. The positions of these fragments from anode to cathode sides of the gel would be

(1) b, a, c, d

(2) a, b, c, d

(3) c, b, a, d

(4) b, a, d, c

Subtopic:  Process of Biotech |
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AIIMS - 2012
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Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be:

A. Denatured double stranded DNA probes
B. doubles stranded RNA probes
C. Protein probes
D. Single stranded DNA probes.

1. A, B
2. B, C
3. A, D
4. A, B, C, D

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A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?

1. Southern blotting and South Western blotting
2. Northern blotting and western blotting
3. Western blotting and Eastern blotting
4. Southern and Northern blotting
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Southern Blotting essentially makes use of a

1. Buffer 2. Electrodes
3. Agarose gel 4. All of the above
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The native charge on DNA molecules is

1. Positive 2. Negative
3. Neutral 4. DNA is an ampholyte
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