A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :
1. ampicillin but not tetracycline
2. tetracycline but not ampicillin
3. both tetracycline and ampicillin
4. neither tetracycline nor ampicillin
Subtopic: Selectable Markers: II | Selectable Markers |
60%
Level 2: 60%+
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A gene, whose expression helps to identify transformed cells is known as
1. selectable marker
2. vector
3. plasmid
4. structural gene
Subtopic: Selectable Markers: II | Selectable Markers |
87%
Level 1: 80%+
NEET - 2017
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How are transformants selected from nontransformants?
| 1. | Presence of more than one recognition site in the vector DNA. |
| 2. | Presence of alien DNA into the vector DNA results into insertional inactivation of selectable marker. |
| 3. | Antibiotic resistance gene gets inactivated due to insertion of alien DNA. |
| 4. | Both 2 and 3. |
Subtopic: Selectable Markers: II | Selectable Markers: III | Selectable Markers |
83%
Level 1: 80%+
AIIMS - 2014
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Why is a marker gene useful?
| 1. | It shows you whether the gene being added, has been taken up or not. |
| 2. | It's a way of labelling which gene you want to modify. |
| 3. | It sticks with the striker gene. |
| 4. | It marks the position of r-DNA |
Subtopic: Selectable Markers: II | Selectable Markers |
70%
Level 2: 60%+
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Transformation is the term used to denote
| 1. | Non virus mediated gene transfer in non Animal eukaryotic cells |
| 2. | Virus mediated gene transfer in Bacteria |
| 3. | Virus mediated gene transfer in non Animal eukaryotic cells |
| 4. | All of the above |
Subtopic: Selectable Markers: II | Selectable Markers |
Level 4: Below 35%
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Selectable marker helps
| 1. | To identify transformants |
| 2. | To identify recombinants |
| 3. | To identify non- recombinants |
| 4. | To eliminate transformants |
Subtopic: Selectable Markers: I | Selectable Markers: II | Selectable Markers |
55%
Level 3: 35%-60%
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