Which of the following restriction sites is located within the gene for tetracycline resistance in the plasmid pBR322?
1. BamHI
2. Psti
3. Clal
4. Pvull
The technique not used for transformation of plant cells in recombinant procedures is:
1. Biolistics
2. Agrobacterium mediation
3. Use of viruses
4. Micro-injection
Microinjection is suitable for :
1. Injecting an ovum into the sperm in IVF
2. Transforming animal cells
3. Injecting very small sized drug particles into neurons
4. Conferring antibiotic resistance to a certain strain of bacteria
The plasmid pBR322 does not contain:
1. An origin of replication site
2. A gene that encodes for restrictor of plasmid copy number
3. Gene for Ampicillin and Streptomycin resistance
4. Sites for many restriction enzymes
A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of:
1. BamH I and Sma I
2. Hind II and Sma I
3. BamH I and SaI I
4. SaI I and Hind II
The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. rop-reduced osmotic pressure
3. Hind III, Eco RI -selectable markers
4. ampR, tetR-antibiotic resistance genes
For transformation, microparticles coated with DNA to be bombarded with gene gun are
made up of
1. silver or platinum
2. platinum or zinc
3. silicon or platinum
4. gold or tungsten
Which one of the following is used as vector for cloning genes into higher organisms?
1. Baculovirus
2. Salmonella typhimurium
3. Rhizopus nigricans
4. Retrovirus
Which one of the following is commonly used in transfer of foreign DNA into crop plants?
1. Trichoderma harzianum
2. Meloidogyne incognita
3. Agrobacterium tumefaciens
4. Penicillium expansum
Microbes found to be very useful in genetic engineering are:
1. Escherichia coli and Agrobacterium tumefaciens
2. Vibrio cholerae and a tailed bacteriophage
3. Diplococcus sp. and Pseudomonas sp.
4. Crown gall bacterium and Caenorhabditis elegans