Identify a character that is not desirable in a cloning vector:

1. an inactive promoter

2. an origin of replication site

3. selectable markers such as genes for antibiotic resistance

4. one or more unique restriction endonuclease sites

The DNA molecule to which the gene of interest is integrated for cloning is called:

Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Which one of the following is a case of wrong matching ?

1. Somatic hybridisation - Fusion of two diverse cells

2. Vector DNA - Site for tRNA synthesis

3. Micropropogation - In vitro production of plants in large numbers

4. Callus - Unorganised mass of cells produced in tissue culture

Which of these are most widely used in genetic engineering?

1. Plastid

2. Plasmid

3. Mitochondrion

4. Ribosome

Plasmid vector in DNA recombinant technology means

1. a virus that transfers gene to bacteria

2. extra-chromosomal autonomously replicating circular DNA

3. sticky end of DNA

4. any fragment of DNA carrying desirable gene

Which of the following statement is not true: 

(1) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium

(2) Normally, the genes encoding resistance to antibiotics are considered useful selectable markers for E.coli

(3)  The normal E.coli cells do not carry resistance against antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin

(4) Presence of one recognition sites within the vector will generate several fragments, which will complicate the gene cloning

To be useful in genetic engineering a vector must have

1. An origin for replication
2. Low copy number
3. Low rates of multiplication
4. Many cloning sites for the same enzyme

A gene has been inserted into a vector for the purpose of cloning the sequence only. This vector is called

a.  Suppression vector

b. Expression vector

c. Cloning vector

d. None of these

Recombinant DNA is formed in nature during-