- 1(current)
Q. No.Transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization is called as:
| 1. | DNA profiling | 2. | PCR |
| 3. | Southern blotting | 4. | Elution |
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What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
| I. | Transform bacteria with recombinant DNA molecule. |
| II. | Cut the plasmid DNA using restriction enzymes. |
| III. | Extract plasmid DNA from bacterial cells. |
| IV. | Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. |
| V. | Use ligase to seal plasmid DNA to non-plasmid DNA. |
1. I, II, IV, III, V
2. II, III, V, IV, I
3. III, II, IV, V, I
4. III, IV, V, I, II
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The "Southern" technique involves:
| 1. | the detection of DNA fragments on membranes by specific radioactive antibodies. |
| 2. | the detection of proteins on membranes using a radioactive DNA probe. |
| 3. | the detection of DNA fragments on membranes by a radioactive DNA probe. |
| 4. | the detection of proteins on membranes using specific radioactive antibodies. |
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A piece of nucleic acid used to find a gene, by forming a hybrid with it, is called a
1. Probe
2. Vector
3. Restriction sequence
4. Retrovirus
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The size of VNTR [used in DNA fingerprinting] varies from:
| 1. | 0.1 to 20 kb | 2. | 20 to 30 kb |
| 3. | 50 to 100 kb | 4. | 115 to 215 kb |
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What is it that forms the basis of DNA Fingerprinting?
| 1. | The relative difference in the DNA occurrence in blood, skin and saliva |
| 2. | The relative amount of DNA in the ridges and grooves of the fingerprints |
| 3. | Satellite DNA occurs as highly repeated short DNA segments |
| 4. | The relative proportions of purines and pyrimidines in DNA |
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- 1(current)
Q. No.
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