- 1(current)
Q. No.How many recognition sequences for the restriction enzyme EcoRI is/are expected to be present in a dsDNA of 10000 base pair length?
1. 0
2. 1
3. 2
4. 4
DNA is a polymer of nucleotides which are linked to each other by 3’-5’ phosphodiester bond. To prevent polymerisation of nucleotides, which of the following modifications would you choose?
1. Replace purine with pyrimidines
2. Remove/Replace 3' OH group in deoxy ribose
3. Remove/Replace 2' OH group with some other group in deoxy ribose
4. Both ‘b’ and ‘c’
Deoxyribonucleotides in a ds DNA are held by:
I. Phosphodiester.
II. Hydrogen.
III. Ionic.
1. I and II only
2. I and III only
3. II and III only
4. I, II and III
What is incorrect regarding the salient features of the DNA double helix?
1. The two chains have anti-parallel polarity
2. The base pairing is complementary
3. Backbone of a strand is constituted by sugar-phosphate
4. The pitch is 0.34 nm
Identify the incorrect statement regarding structure of DNA:
1. Pitch is 3.4nm and 10 base pairs.
2. The two strands are antiparallel.
3. Each pair of nucleotides is held together by three hydrogen bonds.
4. The diameter of the molecule is a uniform 2nm.
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During transcription, if the nucleotide sequence of the DNA strand that is being coded is ATACG, then the nucleotide sequence in the mRNA would be:
1. TCTGG
2. UAUGC
3. UATGC
4. TATGC
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| 1. | The total amount of purine nucleotides and pyrimidine nucleotides is not always equal |
| 2. | There are two strands, which run parallel in the 5' → 3’ direction |
| 3. | The proportion of adenine in relation to thymine varies with the organism |
| 4. | There are two strands, which run antiparallel - one in 5' → 3’ direction and the other in 3’→ 5’ |
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The diagram shows an important concept in the genetic implication of DNA. Fill in the blanks A to C.
1. A - translation B - transcription C - Erwin Chargaff
2. A -transcription B - translation C - Francis Crick
3. A - translation B - extension C - Rosalind Franklin
4. A - transcription B - replication C - James Watson
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In sea urchin DNA, which is double stranded, 17% of the bases were shown to be cytosine. The percentages of the other three bases expected to be present in this DNA are :
1. G 17%, A 16.5%,T32.5%
2. G 17%, A 33%,T 33%
3. G8.5%,A50%, T24.5%
4. G 34%,A 24.5%,T 24.5%
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- 1(current)
Q. No.
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