Q. No.The primary RNA transcript in eukaryotes contains introns. This can be demonstrated by:
I:
A comparison of the DNA and mRNA sequences.
II:
A comparison of the genomic DNA and cDNA sequences.
III:
A hybridization between DNA and mRNA molecules.
1.
I and II only
2.
I and III only
3.
II and III only
4.
I, II, and III
What would be true for the mutation that leads to sickle cell anemia ?
| I: | Is a base substitution. |
| II: | Is a deleterious mutation. |
| III: | Is a missense mutation. |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Expression of the lac operon was completely repressed. This will lead to:
| 1. | The low level of lacZ, lacY, and lacA gene expression will continue. |
| 2. | Allolactose would accumulate within the cell and become toxic. |
| 3. | Lactose would not be converted into the inducer and the operon could not be induced. |
| 4. | The cell will not be able to use glucose as well. |
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What type of changes will lead to a frameshift mutation?
| I: | A transition | II: | A transversion | III: | A deletion of 3 bp |
| 1. | I and II only | 2. | III only |
| 3. | I, II, and III | 4. | None |
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Identify the correct statements regarding mutations:
| I: | Are permanent changes in the DNA sequence or structure. |
| II: | Produce allelic variation. |
| III: | Are more likely to be harmful than beneficial. |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Regulation of gene expression very commonly involves transcriptional regulation. The most important significance of this is that it:
| 1. | is highly efficient at completely preventing transcription. |
| 2. | allows the cell to only produce proteins that are needed at the time. |
| 3. | can be induced by a repressor protein. |
| 4. | can be done only in prokaryotes. |
What would be the actual inducer for the lac operon?
| 1. | lactose | 2. | allolactose |
| 3. | ß-galactosidase | 4. | galactose |
The origin of replication is a particular sequence in a genome at which replication is initiated. This region is AT-rich because:
| 1. | DnaA proteins bind to AT-rich sites. |
| 2. | DnaC proteins bind to AT-rich sites. |
| 3. | AT base pairs are more easily separated than GC base pairs. |
| 4. | AT base pairs contain more hydrogen bonds than GC base pairs. |
Identify the correct statements regarding a replisome:
| I: | It is a complex of several proteins. |
| II: | It includes enzymes like DNA polymerase, DNA helicase, and primase. |
| III: | It is found at the replication fork during DNA synthesis. |
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
T2 bacteriophage was used by Hershey and Chase to conclusively prove that DNA and not protein is the genetic material because:
| 1. | It makes capsomeres with the help of its genes. |
| 2. | It injects its genetic material into a bacterial cell. |
| 3. | It can undergo either the lytic or lysogenic life cycle |
| 4. | It enters the bacterial cell to cause infection. |
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