Q. No.Using reverse transcriptase or gene synthesis has an advantage over the shotgun approach in obtaining a copy of the gene required as:
1.
the gene produced can easily be ligated to the vector
2.
the gene produced is not a split gene
3.
the gene produced does not require a promoter to be expressed
4.
the gene produced does not require an ori to replicate
The promoter site and the terminator site for transcription are located at:
| 1. | 3' (downstream) end and 5' (upstream) end, respectively of the transcription unit |
| 2. | 5' (upstream) end and 3' (downstream) end, respectively of the transcription unit |
| 3. | the 5' (upstream) end |
| 4. | the 3' (downstream) end |
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With regard to mature mRNA in eukaryotes:
| 1. | exons and introns do not appear in the mature RNA. |
| 2. | exons appear but introns do not appear in the mature RNA. |
| 3. | introns appear but exons do not appear in the mature RNA. |
| 4. | both exons and introns appear in the mature RNA. |
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Discontinuous synthesis of DNA occurs in one strand, because:
| 1. | DNA molecule being synthesised is very long |
| 2. | DNA-dependent DNA polymerase catalyses polymerisation only in one direction (5’ → 3’) |
| 3. | it is a more efficient process |
| 4. | DNA ligase joins the short stretches of DNA |
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Which of the following statements is correct about the role of regulatory proteins in transcription in prokaryotes?
| 1. | They only increase the expression |
| 2. | They only decrease the expression |
| 3. | They interact with RNA polymerase but do not affect the expression |
| 4. | They can act both as activators and as repressors |
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While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering Chargaff’s rule it can be concluded that:
| 1. | it is a double-stranded circular DNA |
| 2. | It is single-stranded DNA |
| 3. | It is a double-stranded linear DNA |
| 4. | No conclusion can be drawn |
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DNA fingerprinting is related to:
| 1. | Molecular analysis of profiles of DNA samples |
| 2. | Analysis of DNA samples using imprinting devices |
| 3. | Techniques used for molecular analysis of different specimens of DNA |
| 4. | Techniques used in the identification of fingerprints of different persons |
A nutritionally wild-type organism that does not require additional biochemicals is:
| 1. | Prototroph | 2. | Auxotroph |
| 3. | Phenotype | 4. | Autotroph |
What is it that forms the basis of DNA Fingerprinting?
| 1. | The relative proportions of purines and pyrimidines in DNA. |
| 2. | The relative difference in the DNA occurrence in blood, skin, and saliva. |
| 3. | The relative amount of DNA in the ridges and grooves of the fingerprints. |
| 4. | Satellite DNA occurring as highly repeated short DNA segments |
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Select the correct option:
|
The direction of RNA synthesis |
The direction of reading of the template DNA strand |
|
|
1. |
5' - 3' |
3' - 5' |
|
2. |
3' - 5' |
5' - 3' |
|
3. |
5' - 3' |
5' - 3' |
|
4. |
3' - 5' |
3' - 5' |
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