Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.

Choose the correct answer from the options given below:
1. B, D, A, C 2. B, C, D, A
3. C, A, B, D 4. C, B, D, A
Subtopic:  Process of Biotech |
From NCERT
NEET - 2023
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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism? 

1. Escherichia coli  2. Bacillus thuringiensis 
3. Salmonella typhimurium  4. Agrobacterium tumefaciens 
Subtopic:  Process of Biotech | Introduction & History |
 73%
From NCERT
NEET - 2022
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Spooling is:

1. Amplification of DNA
2. Cutting of separated DNA bands from the agarose gel
3. Transfer of separated DNA fragments to synthetic membranes
4. Collection of isolated DNA 

Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
 71%
From NCERT
NEET - 2020
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Select the correct statement from the following:

1. Gel electrophoresis is used for the amplification of a DNA segment.
2. The polymerase enzyme joins the gene of interest and the vector DNA.
3. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.
4. PCR is used for the isolation and separation of genes of interest.
Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
 73%
From NCERT
NEET - 2020
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Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:

(a) DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal.
(b) DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA.
(c) The smaller the DNA fragment, the greater the distance it travels through it.
(d) Pure DNA can be visualized directly by exposing it to UV radiation.


Choose the correct answer from the options given below:

1. (a), (c) and (d) 2. (a), (b) and (c)
3. (b), (c) and (d) 4. (a), (b) and (d)
Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
From NCERT
NEET - 2019
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Gel electrophoresis is used for:
1. cutting of DNA into fragments
2. separation of DNA fragments according to their size
3. construction of recombinant DNA by joining with cloning vectors
4. isolation of DNA molecule

Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
 90%
From NCERT
AIPMT - 2008
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