Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
1. PsT I
2. Pvu I
3. EcoR I
4. BamH I
Subtopic:  Cloning Vector |
 82%
From NCERT
NEET - 2023
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 Match List - I with List - II
List-I List-II
(A) Kanamycin (I) Delivers genes into animal cells
(B) ClaI (II) Selectable marker
(C) Disarmed retroviruses (III) Restriction site
(D) Kanamycin Rgene (IV) Antibiotic resistance

Choose the correct answer from the options given below:
Options: (A) (B) (C) (D)
1. (II) (III) (I) (IV)
2. (III) (I) (IV) (II)
3. (IV) (III) (I) (II)
4. (II) (IV) (I) (III)
Subtopic:  Cloning Vector |
 51%
From NCERT
NEET - 2023
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Which  of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Subtopic:  Cloning Vector |
 73%
From NCERT
NEET - 2022
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Identify the properties of a good vector used in rDNA technology:

(a) It should have origin of replication supporting a high copy number 
(b) It should have preferably more than '2' recognition sites
(c) The restriction sites in vector should be in the antibiotic- resistant genes 
(d) It should have suitable marker genes 
(e) It should be easy to isolate and purify 


Choose the most appropriate answer from the options given below: 

1. (a), (c) and (e) only  2. (c), (d) and (e) only 
3. (a), (b) and (c) only  4. (a), (c), (d) and (e) only 
Subtopic:  Cloning Vector | Tools |
 86%
From NCERT
NEET - 2022
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Pathogenic bacteria gain resistance to antibiotics due to changes in their :
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
Subtopic:  Cloning Vector |
 83%
From NCERT
NEET - 2022
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Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain,

1. it will lead to the lysis of host cells.
2. it will be able to produce a novel protein with dual abilities. 
3. it will not be able to confer ampicillin resistance to the host cell. 
4. the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside.

Subtopic:  Cloning Vector |
 58%
NEET - 2021
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The two antibiotic resistance genes on vector pBR 322 are for:

1. Ampicillin and Tetracycline

2. Ampicillin and Chloramphenicol

3. Chloramphenicol and Tetracycline

4. Tetracycline and Kanamycin

Subtopic:  Cloning Vector |
 94%
From NCERT
NEET - 2019
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The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)? 
 
1. Ori-original restriction enzyme

2. Rop-reduced osmotic pressure

3. Hind III, Eco RI-selectable markers

4. AmpR, tetiR-antibiotic resistance genes

Subtopic:  Tools: Vectors: II | Cloning Vector | Tools |
 86%
From NCERT
AIPMT - 2012
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Which of the following are used in gene cloning:

1. Lomasomes

2. Mesosomes

3. Plasmids

4. Nucleoids

Subtopic:  Cloning Vector |
 97%
From NCERT
AIPMT - 2010
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In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:

A. a bacterium B. plasmid
C. plasmodium D. bacteriophage

Options:

1. (A) only 2. (A) and (C) only
3. (B) and (D) only 4. (A), (B) and (D) only
Subtopic:  Cloning Vector |
 61%
From NCERT
AIPMT - 2010
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