Upon exposure to UV radiation, DNA stained with ethidium bromide will show: 
1. Bright orange colour
2. Bright red colour
3. Bright Blue colour
4. Bright yellow colour
Subtopic:  Separation and Isolation of DNA fragments |
 91%
From NCERT
NEET - 2023
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Which one of the following statement is not true regarding gel electrophoresis technique?
 
1. Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
2. The process of extraction of separated DNA strands from gel is called elution.
3. The separated DNA fragments are stained by using ethidium bromide.
4. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
Subtopic:  Separation and Isolation of DNA fragments |
 84%
From NCERT
NEET - 2022
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Separation of DNA, fragments is done by a technique known as :
1. Polymerase Chain Reaction
2. Recombinant technology
3. Southern blotting
4. Gel electrophoresis
Subtopic:  Separation and Isolation of DNA fragments |
 90%
From NCERT
NEET - 2022
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Refer to the following statements for agarose-gel electrophoresis:
(a) Agarose is a natural polymer obtained from sea-weed.
(b) The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA.
(c) The DNA migrates from negatively-charged electrode to the positively-charged electrode.
(d) The DNA migrates from positively-charged electrode to the negatively-charged electrode.

Choose the most appropriate answer from the options given below :
1. (a) and (b) only 2. (a), (b) and (c) only
3. (a), (b) and (d) only 4. (b), (c) and (d) only
Subtopic:  Separation and Isolation of DNA fragments |
 82%
From NCERT
NEET - 2022
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DNA strands on a gel, stained with ethidium bromide, when viewed under UV radiation, appear as:

1.Dark red bands2.Bright blue bands
3.Yellow bands4.Bright orange bands

Subtopic:  Separation and Isolation of DNA fragments |
 90%
From NCERT
NEET - 2021
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

1. Histones 2. Polysaccharides
3. RNA 4. DNA

Subtopic:  Separation and Isolation of DNA fragments | General Design of an rDNA experiment |
 87%
From NCERT
NEET - 2021
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Match the following techniques or instruments with their usage:

(a) Bioreactor    (i) Separation of DNA fragments
(b) Electrophoresis  (ii) Production of large
quantities of products
(c) PCR    (iii) Detection of pathogen, based on
antigen-antibody reaction
(d) ELISA    (iv) Amplification of nucleic acids


Select the correct option from the following:

1.  (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
2.  (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
3.  (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i)
4.  (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv)
Subtopic:  Polymerase Chain Reaction: PCR | Bioreactors/Downstream Processing | Separation and Isolation of DNA fragments |
 87%
From NCERT
NEET - 2020
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In a mixture, DNA fragments are separated by-

1. Bioprocess engineering

2. Restriction digestion

3. Electrophoresis

4. Polymerase chain reaction

Subtopic:  Separation and Isolation of DNA fragments |
 91%
From NCERT
NEET - 2020
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Spooling is:

1. Amplification of DNA
2. Cutting of separated DNA bands from the agarose gel
3. Transfer of separated DNA fragments to synthetic membranes
4. Collection of isolated DNA 

Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
 71%
From NCERT
NEET - 2020
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Select the correct statement from the following:

1. Gel electrophoresis is used for the amplification of a DNA segment.
2. The polymerase enzyme joins the gene of interest and the vector DNA.
3. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.
4. PCR is used for the isolation and separation of genes of interest.
Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
 73%
From NCERT
NEET - 2020
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