Thermostable DNA polymerase used in PCR was isolated from:
1. Thermus aquaticus
2. Escherichia coli
3. Agrobacterium tumefaciens
4. Bacillus thuringiensis
Subtopic:  Polymerase Chain Reaction: PCR |
 89%
From NCERT
NEET - 2023
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R) 
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation. 

In the light of the above statements, choose the correct answer from the options given below: 
1. (A) is not correct but (R) is correct. 
2. Both (A) and (R) are correct and (R) is the correct explanation of (A)
3. Both (A) and (R) are correct but (R) is not the correct explanation of (A).
4. (A) is correct but (R) is not correct.
Subtopic:  Selectable Markers: I | Polymerase Chain Reaction: PCR |
 76%
From NCERT
NEET - 2022
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In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to : 
1. High temperature 
2. Two sets of Primers 
3. Taq DNA polymerase 
4. Deoxynucleotides 

Subtopic:  Polymerase Chain Reaction: PCR |
 66%
From NCERT
NEET - 2022
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Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

1. Extension, Denaturation, Annealing

2. Annealing, Denaturation, Extension

3. Denaturation, Annealing, Extension

4. Denaturation, Extension, Annealing

Subtopic:  Polymerase Chain Reaction: PCR |
 83%
From NCERT
NEET - 2021
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During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following PCR will be affected first? 

1. Denaturation 2. Ligation
3. Annealing 4. Extension
Subtopic:  Polymerase Chain Reaction: PCR |
 86%
From NCERT
NEET - 2021
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Match the following techniques or instruments with their usage:

(a) Bioreactor    (i) Separation of DNA fragments
(b) Electrophoresis  (ii) Production of large
quantities of products
(c) PCR    (iii) Detection of pathogen, based on
antigen-antibody reaction
(d) ELISA    (iv) Amplification of nucleic acids


Select the correct option from the following:

1.  (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i)
2.  (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii)
3.  (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i)
4.  (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv)
Subtopic:  Polymerase Chain Reaction: PCR | Bioreactors/Downstream Processing | Separation and Isolation of DNA fragments |
 87%
From NCERT
NEET - 2020
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The Taq polymerase enzyme is obtained from:
1. Thiobacillus ferrooxidans
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
Subtopic:  Polymerase Chain Reaction: PCR |
 94%
From NCERT
NEET - 2016
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Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduces DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperatures

Subtopic:  Polymerase Chain Reaction: PCR |
 77%
From NCERT
AIPMT - 2012
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